青藤碱对兔膝骨关节炎模型组织形态学及关节液炎性细胞和聚集蛋白聚糖酶含量的影响  

Effects of Sinomenine on Morphology and Levels of Inflammatory Cytokines and ADAMTS in Rabbit with Knee Osteoarthritis

作　　者：郑洁[1] 赵莉平[1] 胡亚莉[1] 袁普卫[1] ZHENG Jie;ZHAO Liping;HU Yali;YUAN Puwei(Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学,陕西咸阳712046

出　　处：《中国中医药信息杂志》2019年第6期51-54,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：陕西省科技厅自然科学基础项目(2016JQ8004);陕西省教育厅科研项目(17JK0195);陕西省中医药管理局中医药科研计划项目(JCPT035);陕西省重点科技创新团队项目(2013KCT-26)

摘　　要：目的观察不同剂量青藤碱对兔膝骨关节炎(OA)模型关节软骨和滑膜组织形态学及关节液白细胞介素(IL)-6、IL-17及聚集蛋白聚糖酶(ADAMTS)-4、ADAMTS-5含量的影响。方法采用Hulth法建立OA模型。将50只新西兰大白兔随机分为空白组、模型组和青藤碱低、中、高剂量组。空白组不予任何处理,模型组给予生理盐水、给药组给予盐酸青藤碱注射液膝关节腔注射,共干预10次,青藤碱给药量分别为0.2 mL(5 mg)、0.35 mL(8.75 mg)和0.5 mL(12.5 mg)。干预结束后取关节软骨行番红O染色,取滑膜组织行HE染色,进行组织学评分;ELISA检测关节液IL-6、IL-17、ADAMTS-4和ADAMTS-5含量。结果模型组软骨Mankin’s评分和滑膜组织病理学评分显著高于空白组(P<0.01),青藤碱中、高剂量组软骨Mankin’s评分和滑膜评分显著低于模型组(P<0.05,P<0.01);模型组关节液IL-6、IL-17、ADAMTS-4和ADAMTS-5含量较空白组显著升高(P<0.01),青藤碱中、高剂量组关节液IL-6、IL-17、ADAMTS-4和ADAMTS-5含量较模型组显著降低(P<0.05,P<0.01)。结论中、高剂量青藤碱对OA模型病变软骨具有保护作用。Objective To observe the effects of different concentrations of sinomenine (SIN) on morphology of synovium and cartilage and contents of IL-6, IL-17, ADAMT-4 and ADAMT-5 in synovial fluid of rabbit knee osteoarthritis model. Methods After establishing the knee osteoarthritis model by Hulth method, the New Zealand white rabbits were randomly divided into control group, model group, SIN low-, medium- and high-dosage groups. Control group did not receive any treatment. Model group and other administration group received saline and SIN knee joint injection, for 10 times. The dosages of SIN were 0.2 mL (5 mg), 0.35 mL (8.75 mg), and 0.5 mL(12.5 mg), respectively. After intervention, the articular cartilage was stained with Safranin O, and the synovium was taken for HE staining and histological scoring. The levels of IL-6, IL-17, ADAMTS-4 and ADAMTS-5 in joint fluid were detected by ELISA. Results Mankin’s scores of articular cartilage and histological scores of synovium in model group were significantly higher than control group (P<0.01), and the Mankin's score and synovial score in SIN medium- and high-dosage groups were significantly lower than those in the model group (P<0.05, P<0.01). The levels of IL-6, IL-17, ADAMTS-4 and ADAMTS-5 in the model group were significantly higher than those in the control group (P<0.01). The levels of IL-6, IL-17, ADAMTS-4 and ADAMTS-5 in SIN medium- and high-dosage groups were significantly lower than those in the model group (P<0.05, P<0.01). Conclusion SIN medium and high dosage can delay the degradation of articular cartilage of osteoarthritis model.

关 键 词：青藤碱 骨性关节炎 白细胞介素-6 白细胞介素-17 聚集蛋白聚糖酶 兔 

分 类 号：R285.5[医药卫生—中药学]