槐花饮片标准汤剂研究  

Study on Standard Decoction of Sophorae Flos Decoction Pieces

作　　者：高乐 王晓亚[1,2,3] 王相 王鑫国[1,2,3] 李军山 牛丽颖[1,2,3] GAO Le;WANG Xiaoya;WANG Xiang;WANG Xinguo;LI Junshan;NIU Liying(Hebei University of Chinese Medicine,Shijiazhuang 050091,China;Hebei TCM Formula Granule Engineering & Technology Research Center,Shijiazhuang 050091,China;TCM Formula Granule Research Center of Hebei Province University,Shijiazhuang 050091,China;Shineway Pharmaceutical Group Ltd.,Shijiazhuang 051430,China)

机构地区：[1]河北中医学院,河北石家庄050091 [2]河北省中药配方颗粒工程技术研究中心,河北石家庄050091 [3]河北省高校中药配方颗粒应用技术研发中心,河北石家庄050091 [4]神威药业集团有限公司,河北石家庄051430

出　　处：《中国中医药信息杂志》2019年第6期86-91,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：河北省高等学校科学技术研究项目(ZD2015001);河北中医学院青年科研基金(QNZ2018009)

摘　　要：目的制备槐花饮片标准汤剂,并建立质量控制方法。方法制备15批槐花饮片标准汤剂,以总黄酮和芦丁为检测指标,计算出膏率、指标成分含量和转移率,并建立超高液相色谱(UPLC)特征图谱,采用《中药色谱指纹图谱相似度评价系统(2012版)》软件进行分析和相似度评价。结果 15批槐花饮片标准汤剂出膏率为28.7%～39.7%;总黄酮含量为158.07～210.55mg/g,转移率为49.46%～73.19%;芦丁含量为105.26～127.35 mg/g,转移率为41.30%～75.67%。15批槐花饮片标准汤剂UPLC特征图谱标定了9个共有峰,并指认芦丁1个色谱峰,通过与对照图谱比对,相似度均高于0.99。结论本研究建立了槐花饮片标准汤剂的质量评价方法,且准确、稳定、重复性良好,可为槐花配方颗粒的质量控制提供参考。Objective To prepare standard decoction of Sophorae Flos decoction pieces;To establish the method of quality control. Methods Fifteen batches of standard decoction of Sophorae Flos decoction pieces were prepared. With total favonoids and rutin as the detection index, the extraction rate, content and transfer rate of index components were calculated and the UPLC characteristic chromatograms was established. Similarity Evaluation System for Chromatographic Fingerprint of TCM (2012) was used to analyze and compare the characteristic chromatograms. Results The extractum rate of 15 batches of Sophorae Flos decoction pieces was at the range of 28.7%–39.7%;the content of total favonoids ranged from 158.07 mg/g to 210.55 mg/g and transfer rate ranged from 49.46% to 73.19%;the content of rutin ranged from 105.26 mg/g to 127.35 mg/g and transfer rate ranged from 41.30% to 75.67%. UPLC characteristic chromatograms of 15 batches of standard decoction marked nine common chrmatographic peaks and identified one chromatographic peak of rutin. The similarity was higher than 0.99 by comparison with the control map. Conclusion The quality control method of Sophorae Flos decoction pieces was established. The method is accurate, stable and with good repeatability, which can provide a reference for the quality control of formula granules of Sophorae Flos.

关 键 词：槐花饮片 标准汤剂 配方颗粒 特征图谱 芦丁 

分 类 号：R284.1[医药卫生—中药学]