番茄斑萎病毒系统侵染我国大蒜  

Systemic infection of Chinese garlic by Tomato spotted wilt tospovirus

作　　者：宋晓宇 刘勇 陈建斌[1] 史晓斌 张德咏 SONG Xiaoyu;LIU Yong;CHEN Jianbin;SHI Xiaobin;ZHANG Deyong(Longping Branch,Graduate School of Hunan University, Changsha 410125, China;Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China)

机构地区：[1]湖南大学研究生院隆平分院,长沙410125 [2]湖南省农业科学院植物保护研究所,长沙410125

出　　处：《植物保护》2019年第3期149-151,173,共4页Plant Protection

基　　金：湖南省科技计划项目(2016RS2019);国家自然科学基金(31571981;31571982)

摘　　要：番茄斑萎病毒Tomato spotted wilt tospovirus(TSWV)是严重危害世界经济作物的一种病毒,寄主范围广泛。我们在研究中发现番茄斑萎病毒能侵染我国大蒜Allium sativum L.。利用摩擦接种法将TSWV接种到健康大蒜上,结果显示:接种14 d后大蒜新生叶片出现褪绿和白色斑点症状。ELISA检测显示大蒜叶片汁液与TSWV的单克隆抗体产生血清学反应,采用TSWV N基因的引物对大蒜叶片总RNA进行RT-PCR,结果扩增出约800 bp的条带,在NCBI上BLAST显示与TSWV YN5576的同源性最高,为98.52%。这些数据表明番茄斑萎病毒系统侵染我国大蒜。Tomato spotted wilt tospovirus (TSWV) causes severe damages to economic crops worldwide, and it can infect a wide range of plant hosts. To determine whether TSWV infect Chinese garlic, the extracts of Nicotiana benthamiana leaves that were infected with TSWV were rub-inoculated to garlic plants. Garlic plants showed chlorotic and white spot symptoms on new leaves at 14 d post inoculation. Then the garlic leaves were detected by ELISA and RT-PCR.Positive results were obtained in the Dot-ELISA of the garlic leaf extracts with monoclonal antibody of TSWV. Furthermore, the target fragments of 800 bp were obtained from all infected garlic leaves by RT-PCR using the specific primers for N gene of TSWV. The sequence of the RT-PCR products from infected garlic leave was analyzed at NCBI, and the results showed that it shared 98.52% of nucleic acids with TSWV YN5576 isolate. Therefore, our results showed that Chinese garlic could be infected by TSWV.

关 键 词：番茄斑萎病毒 大蒜 鉴定 RT-PCR 

分 类 号：S436.33[农业科学—农业昆虫与害虫防治]