聊城市仓储玉米籽粒霉烂病原菌的分离与鉴定  

Isolation and identification of pathogens causing stored maize kernel rot in Liaocheng

作　　者：孙华[1] 郭宁[1] 丁梦军 张海剑[1] 石洁[1] 刘树森 SUN Hua;GUO Ning;DING Mengjun;ZHANG Haijian;SHI Jie;LIU Shusen(Key Laboratory of IPM on Crops in NorthernRegion of North China, Ministry of Agriculture, Baoding 071000, China)

机构地区：[1]河北省农林科学院植物保护研究所农业部华北北部作物有害生物综合治理重点实验室河北省农业有害生物综合防治工程技术研究中心,保定071000

出　　处：《植物保护》2019年第3期179-182,195,共5页Plant Protection

基　　金：国家现代农业产业技术体系建设专项(CARS-02)

摘　　要：为了明确聊城市引起仓储玉米籽粒霉烂的病原菌种类,采用形态学和分子生物学相结合的方法对54份样本进行了病原菌分离和鉴定。结果表明,优势病原菌为拟轮枝镰孢Fusarium verticillioides,其次为哈茨木霉复合种Trichoderma harzianum species complex,分离频率分别为31.48%和24.07%,其他病原菌如禾谷镰孢复合种F.graminearum species complex、层出镰孢F.proliferatum、棘孢木霉T.asperellum、黑曲霉Aspergillus niger、黄曲霉A.flavus、草酸青霉Penicillium oxalicum和玉蜀黍丝核菌Rhizoctonia zeae的分离频率分别为9.26%、1.85%、1.85%、5.56%、5.56%、14.81%和3.70%。基于11个哈茨木霉复合种分离物的EF-1α基因序列进行了系统发育分析,结果表明该地区分离到的均为非洲哈茨木霉T.afroharzianum。致病性测定结果显示,非洲哈茨木霉是玉米穗腐病的致病菌且对玉米产量有一定影响。In order to clarify the pathogens of maize kernel rot during storage in Liaocheng city, the fungi in 54 samples were isolated and identified by a combination of morphological and molecular methods. The results showed that the dominant fungus was Fusarium verticillioides , followed by Trichoderma harzianum species complex (THSC), which were isolated by 31.48% and 24.07%, respectively. The isolation frequencies of other fungi such as F.graminearum species complex, F.proliferatum, T.asperellum, Aspergillus niger, A.flavus, Penicillium oxalicum and Rhizoctonia zeae were 9.26%, 1.85%, 1.85%, 5.56%, 5.56%, 14.81% and 3.70%, respectively. Phylogenetic analysis based on the EF-1α sequences of 11 THSC isolate showed that all isolates in this region were T.afroharzianum . Pathogenicity test revealed that T.afroharzianum was the pathogen of maize ear rot and had some negative effect on corn yield.

关 键 词：仓储 籽粒霉烂 病原菌 哈茨木霉 

分 类 号：S432.44[农业科学—植物病理学]