甘肃省大麦条纹病菌遗传多样性及致病力差异  

Genetic diversity analysis and virulence differentiation of Pyrenophora graminea,the pathogen of barley leaf stripe in Gansu province

作　　者：李葆春[1] 司二静[1,2] 杨淑莲[3] 孟亚雄[1,2] 马小乐[1,2] 王化俊[1,2] LI Baochun;SI Erjing;YANG Shulian;MENG Yaxiong;MA Xiaole;WANG Huajun(Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Key Laboratory of Crop Important & Germplasm Enhancement, Lanzhou 730070, China;College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China;Archives of Gansu Agricultural University, Lanzhou 730070, China)

机构地区：[1]甘肃省干旱生境作物学重点实验室甘肃省作物遗传改良与种质创新重点实验室,兰州730070 [2]甘肃农业大学农学院,兰州730070 [3]甘肃农业大学档案馆,兰州730070

出　　处：《植物保护》2019年第3期190-195,共6页Plant Protection

基　　金：甘肃农业大学引进人才专项(GSAU-RCZX201706);甘肃农业大学盛彤笙科技创新基金(GSAU-STS-1735);国家自然科学基金(31171558;31460347);国家现代农业产业技术体系建设专项(CARS-05)

摘　　要：为了解甘肃省大麦条纹病病原菌Pyrenophora graminea的遗传多样性及致病力差异,运用RAPD分子标记技术对大麦条纹病菌不同菌株进行遗传多样性分析,并采用三明治法进行菌株致病力差异研究。结果表明:17个RAPD标记从45个菌株中扩增出126条带,平均每个标记7.41条带,遗传相似系数范围为0.468 3～0.984 1,平均值为0.830 8,当遗传相似系数为0.723 6时,可将供试菌株划分为4个类群,分别包含41、2、1和1个菌株;致病力测定结果显示菌株QWC较菌株QQ致病力强,两菌株除在品种‘甘啤2号’和‘GP-3’上无致病力外,在其他供试品种上致病力均存在差异。表明大麦条纹病菌不同菌株间存在遗传差异,且菌株QWC和菌株QQ存在致病力差异。The genetic diversity of Pyrenophora graminea collected from different regions in Gansu, was detected with RAPD markers, and the virulence difference between isolate QQ and isolate QWC was tested by the sandwich method. The results showed that a total of 126 fragments were obtained from 45 isolates. Among them, all of the fragments were polymorphic, with an average of 7.41 fragments per RAPD marker. The genetic similarity ranged from 0.468 3 to 0.984 1, with the average of 0.830 8. Isolates in the study were classified into 4 groups at a genetic similarity level of 0.723 6. Virulence test showed that isolate QWC had higher virulence than isolate QQ, and isolate QWC and isolate QQ had virulence difference on all barley varieties except ‘Ganpi 2’ and ‘GP-3’, which were immune to isolate QWC and isolate QQ. The results indicated that there was genetic difference among 45 isolates and a clear virulence differentiation between isolate QWC and isolate QQ.

关 键 词：大麦条纹病菌 随机扩增多态性DNA 遗传多样性 致病力差异 

分 类 号：S435.123[农业科学—农业昆虫与害虫防治]