小麦苗期钾效率相关性状的全基因组关联分析  

Genome-wide association analysis for wheat traits related to K-efficiency at seedling stage

作　　者：张艳霞 赵艳艳[1] 郭营[1] 赵岩[1] 李斯深[1] 孔凡美[1] ZHANG Yan-xia;ZHAO Yan-yan;GUO Ying;ZHAO Yan;LI Si-shen;KONG Fan-mei(State Key Laboratory of Crop Biology/National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources,Shandong Agricultural University,Tai’an,Shandong 271018,China)

机构地区：[1]山东农业大学作物生物学国家重点实验室/土肥资源高效利用国家工程实验室

出　　处：《植物营养与肥料学报》2019年第5期699-709,共11页Journal of Plant Nutrition and Fertilizers

基　　金：国家重点研发计划“七大作物育种”重点专项(2017YFD0100600);山东省现代农业产业技术体系小麦产业创新团队项目资助

摘　　要：【目的】深入探究小麦钾效率的遗传基础,定向培育钾高效小麦品种是缓解我国钾肥资源短缺的重要途径。本文拟对不同钾处理条件下小麦苗期钾效率相关性状进行鉴定,并进行差异分子标记关联分析,筛选与钾效率紧密关联的分子标记,为钾效率相关基因的克隆及其在育种中的应用奠定基础。【方法】本研究以不同小麦品种(134份)为试验材料,于2014年和2015年,在山东农业大学温室内进行了营养液培养试验。设置正常供钾和低钾水平两个处理,7cm高、7日龄的幼苗在黑暗中生长28天后收获。将幼苗分为地上部和根部,测定植株干重(生物量)、钾含量,计算植株钾累积量、根冠生物量比和钾累积量比和钾利用效率。分析低钾处理对生物量及钾效率相关性状的影响,并以该群体检测到的15230个差异SNP标记为基础,用TASSEL5.0软件中的GLM模型和MLM模型对钾效率相关性状数据进行标记.性状的关联分析,以确定小麦苗期钾效率稳定关联的分子标记位点。【结果】与正常钾处理相比,低钾处理下植株根、冠及全株钾含量及积累量均显著下降,而根冠生物量比、根冠和全株钾利用率均显著增加。关联分析共获得了1300个关联分子标记,其中1102个标记被定位在19条染色体上,这些分子标记绝大多数仅在特定环境条件下被检测到,三个环境下均可检测到的相对稳定的分子标记位点仅有3个,分别为Excalibur_c14273_1407、Ku_c11150_773、BS00094893_51。同时,试验筛选出4个性状簇集位点标记,这些位点同时与6~7个性状存在显著关联,分别为Excalibur_c8670_972、wsnp_Ex_c12887_20426781、wsnp_Ku_c13311_21255428、IACX5989。【结论】低钾处理对小麦苗期生长及钾效率相关性状遗传控制位点有显著影响,低钾处理下与生物量和钾效率相关性状显著关联的分子标记绝大多数在同一个钾处理环境中被检测到,因此不同钾处理环【Objectives】Breeding wheat with K-high efficiency is an important pathway for alleviating pressure of potash resource shortage in China. In this study, to better understanding the genetic mechanism, the correlated traits of K-efficiency at wheat seedling stage were investigated by different K treatments, and the molecular markers closely related to K-efficiency traits were screened by correlation analyses. The study will provide support for cloning of K efficiency-related genes and breeding of wheat varieties with K-high efficiency.【Methods】Using 134 wheat cultivars as materials, a hydroponics experiment with complete random design in triplicate was conducted in greenhouse at Shandong Agricultural University in 2014 and 2015. The seedlings of 7 cm high and 7 days old of all the cultivars were subjected to low K (LK) and normal K (CK) levels for 28 days in the dark, then harvested and divided into shoot and root. The seedling dry weight (biomass) and K content were analyzed, and the K accumulation, dry biomass ratio of root to shoot (RSDW), root to shoot ratio of K accumulation and K use efficiency were calculated. To investigate the effects of low K treatment on biomass and K-efficiency related traits, the correlation analysis of tested traits based on the 15230 different SNP markers was performed using GLM and MLM models in TASSEL 5.0 to obtain stably and significantly correlated markers/locus for wheat seedlings.【Results】The contents and accumulation of K in roots, shoots and whole seedlings were all significantly decreased, although the root biomass and the K use efficiencies significantly increased at the seedling stage under the low K treatment. A total of 1300 markers were significantly associated with biomass and K-efficiency related traits, of which, a total of 1102 markers were located on the 19 chromosomes, and most of these molecular markers were screened only under specific K treatment conditions, and three loci including Excalibur_c14273_1407, Ku_c11150_773 and BS00094893_51 were identifi

关 键 词：小麦 苗期 钾效率 关联分析 

分 类 号：S512.1[农业科学—作物学]