库区植物塘水生植物附着细菌群落季节演替特征及影响因素  

Seasonal succession characteristics and influencing factors of bacterial community attached to aquatic plants in reservoir plant pond

作　　者：朱亮[1,2] 谈悦 陈琳[2] 赵尚 龙艺晴 杨智波 刘畅 ZHU Liang;TAN Yue;CHEN Lin;ZHAO Shang;LONG Yiqing;YANG Zhibo;LIU Chang(Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes, Hohai University, Nanjing 210098, China;College of Environment, Hohai University, Nanjing 210098, China)

机构地区：[1]河海大学浅水湖泊综合治理与资源开发教育部重点实验室,江苏南京210098 [2]河海大学环境学院,江苏南京210098

出　　处：《河海大学学报（自然科学版）》2019年第3期202-208,共7页Journal of Hohai University(Natural Sciences)

摘　　要：采集天然湿地中的狐尾藻、凤眼莲、睡莲3种水生植物,采用高通量测序分析3种水生植物表面附着细菌群落,探究其细菌群落结构的季节演替,并分析环境因子对群落变化的影响。结果表明:(a)3种水生植物附着细菌主要有蓝细菌门(Cyanobacteria)、变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria),这3种植物表面附着的细菌群落结构在门水平上类似,在属水平上丰富度及演替特征存在差异,主要与植物茎叶面积、植物分泌物质及植物茎叶光合作用强度等因素有关;(b)在属水平上,这3种水生植物附着细菌群落季节演替是具有一定方向性的自发进行的过程,不受外在单一因子的限制,是宿主植物和外部水环境共同作用的结果;(c)通过聚类分析发现,环境变化对附着细菌群落结构的影响程度高于植物种类,环境因子的CCA分析表明温度、pH、TN是水生植物附着细菌群落演替的主要影响因素。Three aquatic plants, Foxtail algae, Hyacinth and Water lily, were collected from natural wetlands, and the bacterial community attached to the surface of each plant was analyzed by the high-throughput sequencing. The seasonal succession of bacterial community structure was explored, and the influence of environmental factors on the change of community was also analyzed. The results show that:(a) Cyanobacteria, Proteobacteria, Bacteroidetes and Actinobacteria were the main bacteria adhering to these aquatic plants. The community structure of bacteria adhering to the surface of the three aquatic plants was similar at the phylum level, while the abundance and succession characteristics were different at the generic level, which are mainly related to the plant stem and leaf area, the plant secretion and the photosynthetic intensity.(b) At the generic level, the seasonal succession of bacterial community attached to three aquatic plants is a spontaneous process with a certain direction, which is not limited by the external single factor and is the affected result of host plant and external water environment.(c) Through the cluster analysis, it was found that the effect of environmental change on the community structure of attached bacteria was higher than that of plant species. CCA analysis of environmental factors showed that temperature, pH and TN were the main factors affecting the succession of attached bacterial community of aquatic plants.

关 键 词：附着细菌群落 季节演替 水生植物 环境因子 聚类分析 CCA 

分 类 号：X524[环境科学与工程—环境工程]