油菜不同根肿病抗性材料的室内鉴定及相关生理分析  

Laboratory identification of different resistant materials with clubroot and related physiological analysis in rapeseed

作　　者：蒋学飞 岳出 刘双双 王茂林 JIANG Xue-Fei;YUE Chu;LIU Shuang-Shuang;WANG Mao-Lin(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of life sciences, Sichuan University, Chengdu 610064, China)

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室

出　　处：《四川大学学报（自然科学版）》2019年第3期573-580,共8页Journal of Sichuan University(Natural Science Edition)

基　　金：“国家重点研发计划”项目子课题(2016YFD010020002);四川省科技成果转化项目(16NZ0065);四川省育种攻关(2016NYZ0031)

摘　　要：结合田间种植的根肿病抗性表现,以菌土法和在蘸根法对多个疑似具有抗性的材料进行室内鉴定,初步筛选出两种对根肿菌4号生理小种具有抗性的油菜材料Kc84-1和OG-13;用病情统计的方法将Kc84-1与目前已推广种植的抗性材料华双5R和易感材料R-197进行对照验证,并对三种材料进行抗性生理分析.结果表明:Kc84-1与华双5R具有相同水平的根肿病抗性;不同材料的防御酶超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性以及抗病相关物质丙二醛(MDA)含量的表现各不相同,两种抗性材料在较高感病等级下的防御酶SOD、POD、PAL活性显著高于感病材料,PPO活性和MDA含量在抗性材料和易感材料之间没有显著差异.通过水培法观测三种材料感病早期的根毛侵染水平,结果表明,易感材料R-197的根毛侵染率在侵染第6 d到第14 d显著高于抗性材料华双5R和Kc84-1.According to the resistance performance of clubroot in the field, two kinds of rapeseed materials, Kc84-1 and OG-13, which were resistant to the No.4 physiological race of Plasmodiophora brassicae, were preliminarily screened out by the bacterial soil method and the dipping root method among the several suspected disease resistant materials. The clubroot resistance of material Kc84-1 was identified by comparing with the resistant cultivar Huashuang 5 R and the susceptible material R-197 by the disease investigation statistics, and the related resistance physiological analysis of three materials was carried out. The results showed that, there was the same level clubroot resistance of Kc84-1 and Huashuang 5 R. The activity of defense enzymes Superoxide Dismutase(SOD), Peroxidase(POD), Polyphenol oxidase(PPO), Phenylalanine ammonialyase(PAL) and the Malondialdehyde(MDA) content of resistance related substances were different in distinct materials. The activities of the two kinds of resistant materials, such as SOD, POD, and PAL, were significantly higher than that of the susceptible material. There was no significant difference in PPO activity and MDA content between resistant materials and susceptible materials. The observation on root hair infection of three materials at an early stage by hydroponics, the results showed that the root hairs infection rate of susceptible material R-197 was significantly higher than that of resistant varieties Huashuang 5 R and material Kc84-1 in the sixth to fourteenth days of infection.

关 键 词：油菜 根肿病 抗性鉴定 抗性生理 根毛侵染 

分 类 号：Q75[生物学—分子生物学]