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作 者:陈浩[1,2] 王昌权 夏涛[2] 李月婷 王永林 黄勇[1] 李勇军[3] 郑林[1] CHEN Hao;WANG Chang-quan;XIA Tao;LI Yue-ting;WANG Yong-lin;HUANG Yong;LI Yong-jun;ZHENG Lin(Guizhou Provincial Key Laboratory of Pharmaceutics/ State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education),Guizhou Medical University;School of Pharmacy,Guizhou Medical University,Guiyang 550004,China)
机构地区:[1]贵州医科大学贵州省药物制剂重点实验室药用植物功效与利用国家重点实验室 [2]贵州医科大学药学院,贵阳550004 [3]贵州医科大学民族药与中药开发应用教育部工程研究中心
出 处:《天然产物研究与开发》2019年第5期772-778,共7页Natural Product Research and Development
基 金:国家自然科学基金(81460630/U1812403);贵州省科技计划(黔科合平台人才[2017]5601;[2016]5613\5677);贵州省教育厅项目(黔教合KY字[2018]050);贵阳市科研创新团队项目(筑科合同[2017]30-29号)
摘 要:为了考查白及有效部位在肠道的可吸收成分及其代谢特征。基于在体肠灌流模型,采用超高效液相色谱-四极杆-飞行时间质谱仪(UPLC-Q-TOF/MS)对收集到的健康SD大鼠循环肠灌流液、血清、胆汁进行分析检测,并结合对照品、质谱碎片信息和Masslynx V4.1工作站中的Single Mass Analysis功能,初步推测吸收和代谢产物的结构式。在大鼠血清和胆汁中,初步鉴定出1,4-二[4-(葡萄糖氧)苄基]-2-异丁基苹果酸、4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯、α-异丁基苹果酸酯原型产物。在大鼠循环肠灌流液、血清和胆汁中,共鉴定出4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯的脱糖后硫酸化代谢产物和二氢菲5的葡萄糖醛酸化代谢产物,其代谢产物主要生水解和葡萄糖醛酸化反应。该方法初步探究了白及有效部位在大鼠循环肠灌流液中可吸收成分和代谢特征,为阐释白及药材的药效物质基础提供实验依据。In order to examine the absorbable components of the Bletilla striata effective parts in the intestine and their metabolic characteristics.Based on the intestine perfusion model,the collected intestinal perfusate,serum and bile of healthy SD rats were analyzed by UPLC-Q-TOF/MS.And combined with the reference substance,MS fragment information and the Single Mass Analysis function in the Masslynx V4.1 workstation,preliminarily speculated the structure of the absorption and metabolites.militarine,gymnoside I andα-isobutylmalic acid were preliminarily identified in serum and bile of rats.A total of gymnoside I and dihydrophenanthrene 5 metabolites were identified in rat circulating intestinal perfusion fluid,serum and bile.The method preliminarily explored the absorbable components and metabolic characteristics of Bletilla striata effective parts in the circulating intestinal perfusate of rats,and provided experimental basis for explaining the pharmacodynamic basis of white and medicinal materials.
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