八种石斛中苯丙醇类和香豆素类化合物含量测定与分析  

Determination and analysis of phenylpropanol and coumarin compounds in eight kinds of Dendrobium

作　　者：师亦洁 周峰旭 李鲜 范宇超 刘宏程[2] SHI Yi-jie;ZHOU Feng-xu;LI Xian;FAN Yu-chao;LIU Hong-cheng(School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University,Kunming 650500,China;Supervision and Testing Center for Farm Product Quality,Ministry of Agriculture,Institute of Quality Standard and Testing Technology,Yunnan Academy of Agricultural Science,Kunming 650223,China)

机构地区：[1]昆明医科大学药学院暨云南省天然药物药理重点实验室,昆明650500 [2]农业部农产品质量监督检验测试中心(昆明)云南省农业科学院质量标准与检测技术研究所,昆明650223

出　　处：《天然产物研究与开发》2019年第5期803-807,819,共6页Natural Product Research and Development

基　　金：国家重点研发计划(2016YFF0201806);云南省科技惠民计划(2014DA001)

摘　　要：新建HPLC双波长同时测定松柏苷、紫丁香苷、东莨菪内酯、滨蒿内酯及香豆素含量的分析方法。采用Shiseido CAPCELL PAK MGⅡC_(18)柱(250 mm×4.6 mm,5μm)分析柱;使用乙腈-水为流动相,梯度洗脱;流速为0.8 mL/min;紫外检测器260 nm,315 nm双波长检测。结果显示,五种成分在测定范围内有良好的线(r^2>0.999);在三个加标浓度下方法回收率为98.7%～102.5%之间,相对标准偏差(RSD)小于2.5%。该方法用于8种石斛品种含量的测定。A new method for simultaneous determination of cedarin,syringin,scutellarin,scoparone and coumarin was devel- oped. The method was analyzed by Shiseido CAPCELL PAK MG II C18 column ( 250 mm × 4. 6 mm,5 μm),and with a gradient solvent system of water-acetonitrile. The flow was set at 0. 8 mL/min. The UV detection was set with double wave- lengths of 260 nm and 315 nm. The method was validated and found to be satisfactorily linear ( r2 > 0. 999). Recoveries ranged from 98. 7% to 102. 5% for all compounds at three different spiking levels and relative standard deviation ( RSD) was with 2. 5% or less. The established method was successfully used for evaluating the quality in eight kinds of Dendrobium.

关 键 词：石斛 苯丙醇类 香豆素类 HPLC 

分 类 号：R284.2[医药卫生—中药学] R932[医药卫生—中医学]