基于UPLC-Q-TOF-MS法分析百合珠芽化学成分及其薯蓣皂苷元抗肿瘤活性研究  

Rapid analysis of chemical constituents of Lily Bulbil by UPLC-Q-TOF-MS and the antitumor activity of diosgenin

作　　者：袁志鹰[1,2] 罗林明 陈乃宏[1,2,3] 梁晟[4] 周小江 黄惠勇 陈妍羽[1] YUAN Zhi-ying;LUO Lin-ming;CHEN Nai-hong;LIANG Sheng;ZHOU Xiao-jiang;HUANG Hui-yong;CHEN Yan-yu(Hunan University of Chinese Medicine;Hunan Engineering Technology Center of Standardization and Function of Chinese Herbal Decoction Pieces,Changsha 410208,China;Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China;Hunan institute for drug control,Changsha 410001,China)

机构地区：[1]湖南中医药大学 [2]湖南省中药饮片标准化与功能工程技术中心,长沙410208 [3]中国医学科学院北京协和医学院药物研究所,北京100050 [4]湖南省药品检验研究院,长沙410001

出　　处：《天然产物研究与开发》2019年第5期808-813,831,共7页Natural Product Research and Development

基　　金：湖南省自然科学基金青年项目(2019JJ50446);国家自然科学基金面上项目(81403206);长沙市科技局项目(kq1801044);湖南省中药饮片标准化及功能工程技术研究中心开放基金(2018-02-01);中央引导地方科技发展专项资金(2019XF-5024)

摘　　要：为建立超高效液相色谱-四级杆飞行时间质谱(UPLC-Q-TOF-MS)快速鉴定百合珠芽中10种化学成分的分析方法,并对薯蓣皂苷元进行抗肿瘤研究。实验采用Thermo Hypersil Gold C_(18)柱(100 mm×2.1 mm,1.9μm);乙腈(A)-0.5%乙酸(B)为流动相,梯度洗脱;电喷雾离子源(ESI),正负离子同步监测。采用CCK8法进行薯蓣皂苷元抗肺肿瘤A549细胞、胃肿瘤HGC-27细胞的药效筛选。结果表明,通过二级高分辨质谱分析结合相关文献,共鉴定对香豆酸、薯蓣皂苷、槲皮素等10个化合物。药理研究显示,薯蓣皂苷元对肺癌A549细胞增殖只有微弱的抑制活性,而对胃癌HGC-27细胞增殖表现出较强的抑制作用。本试验证实UPLC-Q-TOF-MS/MS可从保留时间、紫外吸收光谱、精确相对分子质量、分子式和二级结构碎片等方面对百合珠芽的主要成分进行定性分析,为寻找百合珠芽中活性物质提供一种快速准确的分析方法,且揭示百合珠芽中鉴定得到的单体成分可能具有较强的抗肿瘤活性。To establish a method for rapid identification of 10 components in bulbil of Lilium lancifolium Thunb.by UPLC-Q-TOF-MS,and carry out research of tumor cell proliferation study of diosgenin.A Thermo Hypersil Gold C18 column(100 mm×2.1 mm,1.9 μm) was used with the mobile phase of acetonitrile(A)-0.5% acetic acid(B) by gradient elution.Positive and negative ions were simultaneously monitored by electro-spray ionization(ESI),and CCK-8 assay was used to screen the effect of diosgenin on lung cancer A549 cells and gastric cancer HGC-27 cells.Based on the fragmentation pattern and reference results,the structures of 10 compounds including p-coumaric acid,diosgenin and quercetin were identified.The pharmacological study showed that diosgenin had only a weak inhibitory activity on the proliferation of A549 cells in lung cancer,and had a strong inhibitory effect on the proliferation of HGC-27 cells in gastric cancer.These results suggested that UPLC-Q-TOF-MS/MS can be used in the qualitative analysis of the main components of bulbil of Lilium lancifolium Thunb.from retention time,UV absorption spectrum,precise relative molecular weight,molecular formula and secondary fragment ions,and so on.Meanwhile,diosgenin have a strong inhibitory effect on the proliferation of gastric cancer HGC-27 cells.This research provided a scientific basis for the ues of bulbil of Lilium lancifolium Thunb1..

关 键 词：超高效液相色谱-四级杆飞行时间质谱法 百合珠芽 薯蓣皂苷元 抗肿瘤 

分 类 号：R284.1[医药卫生—中药学] R93[医药卫生—中医学]