紫锥菊提取物及酚酸化合物体外抗血小板聚集活性研究  

Antiplatelet aggregation activities of phenolic compounds in Echinacea purpurea and its extract in vitro

作　　者：齐海艳 陈新蕊 王诗寒 杜妍[2] 裴月湖[1,2] 甘春丽 QI Hai-yan;CHEN Xin-rui;WANG Shi-han;DU Yan;PEI Yue-hu;GAN Chun-li(School of Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;School of Pharmacy,Harbin Medical University,Harbin 150081,China)

机构地区：[1]沈阳药科大学中药学院,沈阳110016 [2]哈尔滨医科大学药学院,哈尔滨150081

出　　处：《天然产物研究与开发》2019年第5期850-857,共8页Natural Product Research and Development

基　　金：黑龙江省高等教育学会青年专项(16Q070);黑龙江省卫生计生委科研课题(2018491);黑龙江省博士后资助经费(LBH-Z18105)

摘　　要：为探究紫锥菊提取物及酚酸化合物体外抗血小板聚集活性,体外抗血小板聚集实验采用Born比浊法,以聚集抑制率和半数抑制浓度为指标评价。同时采用分子对接方法,选择凝血因子V(F5)、凝血因子VIII(F8)及凝血因子XI(F11)与酚酸类化合物进行虚拟对接,研究其抗血小板聚集的分子作用靶点。结果表明化合物S-1～S-10及其提取物对体外ADP诱导的血小板聚集有抑制作用,抑制率呈浓度依赖性,S-6与靶点的结合位点更多,选择性更强。紫锥菊中酚酸类化合物及其提取物在体外均显现出抗ADP诱导的血小板聚集活性,为紫锥菊体内研究提供依据。Born turbidimetry was used to evaluate the aggregation inhibition rate and the half-inhibition concentration. The phenolic acid compounds in Echinacea and its extracts were resistant to platelet aggre-gation induced by 4,5-adenosine diphosphate ( ADP). Using molecular docking method,coagulation factor V ( F5),factor VIII ( F8) and factor XI ( F11) were selected for virtual docking with phenolic compounds to study their molecular targets for anti-platelet aggregation. The re- sults showed that the 60% ethanol ext-ract of Echinacea had inhibitory effect on ADP-induced platelet aggregation in vitro, and the inhibition rate was concentration-dependent. The chemically synthesized phenolic compounds all have anti-ADP-in duced platelet aggregation activity,and the anti-platelet aggregation effects of three compounds were similar to those off ASA against platelet aggregation. Molecular docking studies showed that all compounds can be hydrogen bonded to the target com- pound,wherein the binding energy of the target to some of the co-mpounds can be higher than 50% of the positive control. It was suggested that these may participate in the process of platelet aggregation and exert an inhibitory effect. Compound S-6 had more binding site with the target,more tight binding,stronger binding ability and better selectivity,and was expected to be a lead against platelet aggregation. The phenolic acids and their extracts in Echinacea showed anti-ADP-induced platelet ag- gregation activity in vitro,which provided a basis for the in vivo study of Echinacea.

关 键 词：紫锥菊 酚酸类化合物 凝血因子 虚拟分子对接 抗血小板聚集 

分 类 号：R285.5[医药卫生—中药学]