黔产毛蒟挥发油对油酸致大鼠急性肺损伤的影响及其机制  

The influence of volatile oil of Piper puberulum from Guizhou in oleic acid-induced acute lung injury and its mechanism

作　　者：丁雁南[1,2] 杨艳[2] 李姣 DING Yan-nan;YANG Yan;LI Jiao(School of pharmacy,Zunyi Medical University,Zunyi 563099,China;Department of pharmacy Affiliated Hospital of Zunyi Medical College,Zunyi 563003,China)

机构地区：[1]遵义医学院药学院,遵义563099 [2]遵义医学院附属医院药剂科,遵义563003

出　　处：《天然产物研究与开发》2019年第5期864-869,752,共7页Natural Product Research and Development

基　　金：贵州省中医药管理局中医药;民族医药科学技术研究课题(QZYY-2016-009)

摘　　要：为研究黔产毛蒟挥发油在油酸诱导的大鼠急性肺损伤中的作用及其机制。实验将雄性成年清洁级SD大鼠按照体重随机分为对照组、油酸模型组和毛蒟挥发油组(0.125、0.25、0.5 mL/kg)。油酸模型组大鼠采用右侧颈静脉注射油酸0.2 mL/kg形成急性肺损伤模型;毛蒟挥发油组大鼠在油酸造模前30分钟静脉注射毛蒟挥发油;建模4 h后处死,留取标本。观察各组肺组织病理学形态并进行肺损伤评分,同时测定血气分析值、右下肺湿干重、肺通透指数以及肺泡灌洗液中TNF-α、IL-6和IL-1β炎症因子的含量,最后采用免疫组化和Western Blot检测p38MAPK和p-p38MAPK蛋白的表达量。结果表明大鼠PaO_2和PaO_2/FiO_2在油酸模型组明显低于对照组,同时右下肺湿干重、肺通透指数以及肺泡灌洗液中TNF-α、IL-6和IL-1β炎症因子的含量在油酸模型组明显高于对照组。油酸模型组肺组织病理学显示肺损伤明显;毛蒟挥发油组上述指标较油酸模型组明显减轻。p-p38MAPK蛋白表达量在油酸模型组中明显高于对照组,而p-p38MAPK蛋白表达量在毛蒟挥发油组中明显低于油酸模型组。实验证明黔产毛蒟挥发油能够通过抑制p38MAPK通路减少急性肺损伤炎症因子的产生,对急性肺损伤具有较好的保护作用。To investigate the influence of volatile oil of Piper puberulum from Guizhou in oleic acid-induced acute lung injury and its mechanism.Healthy adult male SD rats were randomly divided into three equal groups:normal control group,oleic acid model group and volatile oil group(0.125 mL/kg,0.25 mL/kg and 0.5 mL/kg).Oleic acid model group was administered oleic acid(0.2 mL/kg) via right jugular vein;In volatile oil group,volatile oil group was injected via jugular vein,followed 30 min before by oleic acid infusion;At the 4 hours animals were sacrificed,pathological changes of the lung tissue were examined with light microscope.Arterial blood gas,the wet/dry weight(W/D) of the right lower lung were examined,lung index,LPI and Levels of TNF-α,IL-6 and IL-1β in BALF were examined.The expression conditions of p38 MAPK and p-p38 MAPK were determined by Western blot and immunohistochemical method.The results show that compared to control group,PaO2 and PaO2/FiO2 declined in the animals of oleic acid model group,while right lower lung wet/dry ratio,lung index,LPI and Levels of TNF-α,IL-6 and IL-1β in BALF were increased obviously.Pulmonary edema was observed in injured lung pathology.Compared to oleic acid model group,those indexes were improved in volatile oil group.The lung tissue levels of p-p38 MAPK expressions were markedly higher in oleic acid model group compared to control group.Compared to oleic acid model group,the expression of p-p38 MAPK was reduced in volatile oil group.Experiments have proved that volatile oil of Piper puberulum from Guizhou has a better protection effect to acute lung injury and reduces the cytokine production and it can exert its action by inhibiting p38 MAPK pathway.

关 键 词：黔产毛蒟挥发油 油酸 急性肺损伤 炎性 P38MAPK 

分 类 号：R966[医药卫生—药理学]