有机溶液环境中的迷迭香酸纳滤分离行为及富集工艺  

Separation behavior and enrichment process of rosmarinic acid from low concentration ethanol-aqueous solution by nanofiltration

作　　者：李存玉[1,2] 马赟 李红阳[1] 彭国平[1,2] LI Cun-yu;MA Yun;LI Hong-yang;PENG Guo-ping(College of Pharmacy,Nanjing University of Chinese Medicine;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing 210023,China)

机构地区：[1]南京中医药大学药学院 [2]江苏省中药资源产业化过程协同创新中心,南京210023

出　　处：《天然产物研究与开发》2019年第5期895-901,共7页Natural Product Research and Development

基　　金：国家自然科学基金(81503258;81603307);江苏省高校自然科学基金(17KJB360010);国家中药标准化行动计划(ZYBZH-C-JS-34)

摘　　要：本文采用纳滤分离低浓度乙醇水溶液中迷迭香酸,分析其分离行为并探索富集可行性。结果表明:乙醇浓度对纳滤膜通量和迷迭香酸截留率均有影响,通过响应面法建立二次回归模型,在保障分离效率及截留率的前提下,优化参数为溶质浓度0.41 mg/mL,乙醇浓度20.00%,截留分子量450 Da,迷迭香酸截留率93.81%,预测值较接近与理论值误差较小,数学模型准确可行。通过工艺对比,纳滤富集工艺相较于传统减压浓缩,迷迭香酸保留率提高35.1%,技术优势明显。结果还表明,乙醇水溶液环境下,长周期使用纳滤膜存在溶胀或污染的不可逆性,迷迭香酸截留率未发生明显变化,说明纳滤膜组件分离性能的耐用性较好。In this study,the separation rule and the enrichment feasibility of nanofiltration was explored to separate rosmarinic acid from low concentration ethanol-aqueous solution.The experiment showed that ethanol concentration had influences on the membrane flux and the rejection of rosmarinic acid.In order to ensure the separation efficiency and rejection,the optimal conditions were optimized by response surface method of quadratic regression model,and the results were 0.41 μg/mL for rosmarinic acid concentration,20% for ethanol concentration and 450 Da for molecular weight cut-off.Under these conditions,the rejection of rosmarinic acid was 93.81%,which was close to the predicted values.These results indicated the quadratic polynomial mathematical model built by Design-Expert software was accurate and feasible and the optimized concentrate process was stable and reliable.In addition,the results showed that nanofiltration had several big advantages over the vacuum concentrate.When the nanofiltration membrane was applied in ethanol-aqueous solution for long time,the membrane fouling and swelling was not reversible.But the rejection of rosmarinic acid was stable,which suggested that the nanofiltration membrane module was of better durability.

关 键 词：迷迭香酸 乙醇 纳滤 减压浓缩 耐用性 

分 类 号：R943[医药卫生—药剂学]