上海口岸截获的粉蝇属DNA条形码鉴定及系统发育研究  

DNA BARCODING AND PHYLOGENETIC RELATIONSHIP OF PARTIAL SPECIES OF POLLENIA INTERCEPTED AT SHANGHAI PORT

作　　者：杜鹃 邓耀华 张子龙[1] 赵彤言 李深伟[1] 田桢干[1] DU Juan;DENG Yao-Hua;ZHANG Zi-Long;ZHAO Tong-Yan;LI Shen-Wei;TIAN Zhen-Gan(Shanghai International Travel Healthcare Center,Shanghai 20335,China;Shanghai Pudong International Airport Customs,Shanghai 201202,China;State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,Beijing 100071,China)

机构地区：[1]上海国际旅行卫生保健中心,上海200335 [2]上海浦东国际机场海关,上海201202 [3]军事科学院军事医学研究院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071

出　　处：《寄生虫与医学昆虫学报》2019年第1期41-46,共6页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家质检总局科技计划项目(2017IK166);国家质检总局科技计划项目(2016IK219)

摘　　要：使用传统形态学分类方法进行干制标本的物种鉴定通常比较困难。本文按照《国境口岸医学媒介昆虫DNA条形码鉴定操作规程》对上海口岸截获的粉蝇属干制标本进行DNA条形码研究。经BOLD System v4比对,结果显示所得序列分别属于5个种:粗野粉蝇Pollenia rudis、伪粗野粉蝇P.pediculata、游荡粉蝇P.vagabunda、匈牙利粉蝇P.hungarica和Pollenia sp.。引入1条COⅠ序列作为外群对全部序列构建系统发育树(NJ树),结果显示不同物种各自聚为一支。因此,COⅠ条形码技术可以弥补传统形态学分类方法的不足,提高蝇类干制标本鉴定的成功率。Species identification with dry specimen is usually difficult by traditional taxonomy method. According to the operational regulations for DNA barcode identification of medical insects at frontier ports, DNA barcodes of 31 dry specimens of Pollenia species captured at Shanghai port were generated. After comparing with the Barcode of Life Data(BOLD) system v4, all the 31 DNA barcodes are assigned into 5 species: Pollenia rudis(Fabricius, 1794), Pollenia pediculata(Macquart, 1834), Pollenia vagabunda(Meigen, 1826), Pollenia hungarica(Rognes, 1987) and Pollenia sp.. A neighbor-joining tree was created based on the 31 barcodes of Pollenia and an inductive COⅠ sequence as an outgroup, it is revealed that these species can be differentiated by DNA barcodes. Therefore, COⅠ barcoding can overcome the shortages of traditional taxonomy, and improve the success rate in identification of flies with dry specimen.

关 键 词：COⅠ基因 DNA条形码 粉蝇属 国境口岸 

分 类 号：R384[医药卫生—医学寄生虫学]