经皮导管引导下胃左动脉栓塞术对单纯性肥胖比格犬体质量及Ghrelin蛋白、Leptin蛋白表达的影响研究  

Impact of Percutaneous Catheter-guided Left Gastric Artery Embolization on Body Weight,Expressions of Ghrelin Protein and Leptin Protein in Beagle Dogs with Simple Obesity

作　　者：陈伟[1] 朱帝文[1] 纪卫政[1] 顾俊鹏[1] 张海潇[1] 鲍应军 李文栋 任伟新[1] CHEN Wei;ZHU Diwen;JI Weizheng;GU Junpeng;ZHANG Haixiao;BAO Yingjun;LI Wendong;REN Weixin(Department of Interventional Radiology,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院介入放射科

出　　处：《实用心脑肺血管病杂志》2019年第4期42-46,共5页Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease

基　　金：国家自然科学基金(新疆联合基金培育项目)(U1603127)

摘　　要：背景肥胖是一种环境和遗传等多因素引起的慢性代谢性疾病,目前胃左动脉栓塞术治疗肥胖的有效性及可行性正处于研究阶段,但其减重原理尚未完全明确。目的探讨经皮导管引导下胃左动脉栓塞术对单纯性肥胖比格犬体质量及Ghrelin蛋白、Leptin蛋白表达的影响。方法本实验于2018年5-9月完成。选取15只健康成年雄性比格犬随机分为对照组(n=5)和肥胖组(n=10)。对照组比格犬进食普通饲料,肥胖组比格犬参照营养性肥胖动物造模方式,进食高脂饲料;两组比格犬均连续饲养6周。去除对照组和肥胖组内体质量最低和最高的比格犬各2只以缩小误差,然后选取6只肥胖比格犬随机分为肥胖栓塞组和肥胖非栓塞组,每组3只。对照组和肥胖非栓塞组比格犬经导管给予0.9%氯化钠溶液行胃左动脉灌注,肥胖栓塞组比格犬经导管给予聚乙烯醇颗粒行胃左动脉栓塞术。测量并记录各组比格犬术前及术后1、2、3、4、5、6、7、8、9、10周空腹体质量,计算体质量变化百分比;采用Western blot法检测各组比格犬术后10周Ghrelin蛋白、Leptin蛋白表达情况。结果(1)对照组和肥胖非栓塞组比格犬术后10周内体质量变化百分比低于肥胖栓塞组(P<0.05)。(2)术后10周对照组和肥胖非栓塞组比格犬Ghrelin蛋白、Leptin蛋白相对表达量高于肥胖栓塞组(P<0.05)。结论经皮导管引导下胃左动脉栓塞术可通过抑制Ghrelin蛋白、Leptin蛋白表达而影响单纯性肥胖比格犬食欲,进而降低体质量。Background Obesity is a chronic metabolic disease caused by environmental,genetic and other factors,as a treatment for obesity,the effectiveness and feasibility of left gastric artery embolization is under research,but its mechanism action of weight loss is not yet fully clear. Objective To investigate the impact of percutaneous catheter-guided left gastric artery embolization on body weight,expressions of Ghrelin protein and Leptin protein in beagle dogs with simple obesity.Methods This experiment was implemented from May to September 2018. A total of 15 healthy adult male beagle dogs were randomly divided into control group(n=5)and obesity group(n=10). Dogs in control group fed with regular feed,while dogs in obesity group fed with high-fat feed according to modeling methods for nutritionally obese animals;both groups fed for6 weeks. Two beagle dogs with the lowest or highest body mass in control group and obese group were excluded to reduce the error,respectively,a total of 6 obese dogs in obesity group were randomly divided into embolized and non-embolized obesity subgroups,with 3 dogs in each subgroup. Dogs in control group and non-embolized obesity subgroup were treated with left gastric artery perfusion of 0.9% sodium chloride solution,while dogs in mbolized obesity subgroup were treated with left gastric artery perfusion of polyvinyl alcohol particles. Empty stomach weight was measured and recorded before operation,1 week,2 weeks,3 weeks,4 weeks,5 weeks,6 weeks,7 weeks,8 weeks,9 weeks and 10 weeks after operation to calculate the percent change of body weight within 10 weeks after operation;Western blot was used to detect expressions of Ghrelin protein and Leptin protein 10 weeks after operation. Results(1)Percent change of body weight within 10 weeks after operation in embolized obesity subgroup was statistically significantly was greater than that in non-embolized obesity subgroup and control group,respectively(P<0.05).(2)Relative expression quantity of Ghrelin protein and Leptin protein in embolized ob

关 键 词：肥胖症 胃左动脉栓塞术 体质量 Leptin蛋白 Ghrelin蛋白 动物实验 

分 类 号：R723.14[医药卫生—儿科]