腹腔注射N-甲基-D-天冬氨酸对母鼠发情率、早期胚胎及胚子数的影响  

Effects of Intraperitoneal Injection of N-methyl-D-aspartic Acid on Estrus Rate and Number of Early Embryos and Fetuses in Female Mice

作　　者：王启航 尕玉玲 杨泽 李倩 雷晓雪 杨宝山 赵国栋[1] WANG Qi-hang;GA Yu-ling;YANG Ze;LI Qian;LEI Xiao-xue;YANG Bao-shan;ZHAO Guo-dong(College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China;Bureau of Animal Husbandry andVeterinary Medicine of Minle County of Gansu Province,Minle 734500,China)

机构地区：[1]新疆农业大学动物科学学院,新疆乌鲁木齐830052 [2]甘肃省民乐县畜牧兽医局,甘肃民乐734500

出　　处：《畜牧与饲料科学》2019年第5期6-9,共4页Animal Husbandry and Feed Science

基　　金：大学生创新项目(dxscx2018046)

摘　　要：为研究腹腔注射N-甲基-D-天冬氨酸(NMDA)对母鼠发情率和早期胚胎及胚子数的影响,试验选取40只昆明小鼠[(26±1.37)g],随机分为对照组和试验组(每组各20只),对照组腹腔注射0.2mL生理盐水,试验组腹腔注射0.2mLNMDA溶液,采用棉签蘸取母鼠阴道黏液,涂片后用苏木精(HE)染色,判定母鼠发情情况。随后在发情母鼠与公鼠合笼后第3天,每组随机取10只颈椎脱臼法处死小鼠,冲胚后在体视镜下捡胚观察胚胎个数;第9天处死剩余小鼠,取出子宫统计胚子数。试验结果显示,腹腔注射NMDA试验组母鼠发情率、胚胎数和胚子数分别比对照组提高28.57%、18.18%和12.94%。该试验表明腹腔注射NMDA可促进母鼠的发情,提高早期胚胎和胚子数,为NMDA在动物繁殖中的应用提供了理论基础。This experiment was conducted to study the effect of intraperitoneal injection of N-methyl-D-aspartic acid (NMDA) on estrus rate and number of early embryos and fetuses in female mice. A total of 40 Kunming mice weighing (26±1.37)g were selected and randomly allocated into the following two groups: a control group (n=20) received intraperitoneal injection of 0.2 mL normal saline, and an experimental group (n=20) received intraperitoneal injection of 0.2 mL NMDA solution. The vaginal mucus was sampled by dipping with a cotton swab. The smeared samples were stained with HE staining method to determine the estrus of the mice. On the 3rd day after the mating of estrus female mice and male mice, 10 mice were randomly selected from each group and sacrificed by cervical dislocation. After embryos flush, the number of embryos was observed under the stereomicroscope. On the 9th day after the mating, the remaining mice were sacrificed and their uteri were collected to count the number of fetuses. The results showed that compared to the control group, the estrus rate, embryo number and fetuses number of the mice in NMDA group were increased by 28.57%, 18.18% and 12.94%, respectively. In conclusion, intraperitoneal injection of NMDA can promote the estrus of female mice and increase the number of early embryos and fetuses, which provides a theoretical basis for the application of NMDA in animal reproduction.

关 键 词：N-甲基-D-天冬氨酸 小鼠 发情率 胚子数 

分 类 号：S812[农业科学—草业科学]