圈养丹顶鹤血变原虫的流行调查研究  

Investigation on prevalence of Haemoproteus in captive red crowned crane

作　　者：贾婷[1,2] 郑常明[2] 丁楠[2] 杨明海[2] 赵素芬[2] 林宝庆[3] 张显光 罗毅[2] 张成林[2] 索勋[1] JIA Ting;ZHENG Chang-ming;DING Nan;YANG Ming-hai;ZHAO Su-fen;LIN Bao-qing;ZHANG Xian-guang;LUO Yi;ZHANG Cheng-lin;SUO Xun(College of Veterinary Medicine , China Agricultural University, Beijing 100193 , China;Beijing Key Laboratoryof Captive Wildlife Technologies , Beijing Zoo , Beijing 100044 , China;Xianghai National Nature Reserve Administration ,Tongyu 137215 , China;Zhalong National Nature Reserve Administra , Qiqihar 161002 , China)

机构地区：[1]中国农业大学动物医学院,北京海淀100193 [2]北京动物园圈养野生动物技术北京市重点实验室,北京西城100044 [3]吉林向海国家级自然保护区管理局,吉林通榆137215 [4]黑龙江扎龙国家级自然保护区管理局,黑龙江齐齐哈尔161002

出　　处：《中国兽医杂志》2019年第2期3-7,I0003,共6页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金面上项目(31470460);北京市公园管理中心项目(ZX2013019)

摘　　要：本研究于2013年1月至2015年10月采集3个鹤类饲养单位81份丹顶鹤样品、37种245份周边环境的其他鸟类样品,3个单位4种昆虫3948只进行圈养丹顶鹤血变原虫的流行调查研究。采用瑞-姬氏涂片染色镜检以及套式PCR检测血孢子虫cytb基因、测序分析比对等方法调查各地丹顶鹤血变原虫的流行情况。丹顶鹤样品中共检出血变原虫进化支2种,检出率为19.75%,其他鸟类样品的检出率为0.41%,吸血昆虫样品的检出率为1.05%;扎龙、向海的丹顶鹤和环境吸血昆虫、北京的鹈鹕感染同一个血变原虫进化支,并且该进化支是各类动物中检出率最高的血变原虫进化支。In the present study,81 samples of red crowned crane, 245 samples of 37 species of environment birds, and 3 948 samples of 4 species of environment blood sucking insects were collected in 3 crane breeding bases, including Beijing zoo, Xianghai National Nature Reserve, and Zhalong National Nature Reserve. To investigate the prevalence of Haemoproteus, the blood smears were stained with Wright - Giemsa and observed under an optical microscope,cytb gene was amplified by nested PCR,sequenced, and analyzed by BLAST. The results showed that 2 Haemoproteus lineages were found in red crowned crane samples with the relevance ratio of 19. 75%;the relevance ratio of environment birds was 0. 41%, and environment blood sucking insects was 1. 05% Further, a same lineage was detected in red crowned crane, blood sucking insects were in Xianghai and Zhalong, and pelican was in Beijing. The Haemoproteus lineage has the highest relevance ratio among all kinds of animals.

关 键 词：丹顶鹤 血变原虫 流行 调查 

分 类 号：S852.72[农业科学—基础兽医学]