重组禽流感病毒(H5+H7)二价灭活疫苗对鸡和鸭的免疫效果观察  

Immune Effects of the Inactivated H5 and H7 Subtype Avian Influenza Virus Vaccine (H5N1 Re-8 and H7N9 Re-1) Applied in Chickens and Ducks

作　　者：冯肖肖 吴赟竑 吴雪军[1] 华炯钢[2] 张存[2] 张璐 何杰 徐辉[1] FENG Xiao-xiao;WU Yun-hong;WU Xue-jun;HUA Jiong-gang;ZHANG Cun;ZHANG Lu;HE Jie;XU Hui(Zhejiang Center of Animal Disease Control, Hangzhou 311199, China;Zhejiang Academy of Agricultural Sciences,Institute of Animal Husbandry and Veterinary Science, Hangzhou 311199, China)

机构地区：[1]浙江省动物疫病预防控制中心,浙江杭州311199 [2]浙江省农业科学院畜牧兽医研究所,浙江杭州311199

出　　处：《中国兽医杂志》2019年第2期8-14,共7页Chinese Journal of Veterinary Medicine

基　　金：浙江省“三农六方”科技协作项目(CTZB-F16-0728AWZ-SNY1-39)

摘　　要：H7N9流感变异毒株的出现给我国家禽养殖业造成了严重的疫情风险。为了解和评估新型重组禽流感H5+H7二价灭活疫苗(H5N1Re-8株+H7N9H7-Re1株)的免疫效果和抗体消长规律,我们开展了鸡、鸭现场免疫试验。监测结果表明,该疫苗对蛋鸡和蛋鸭都具有良好的免疫效果,H5和H7亚型免疫抗体水平在免疫后2周均能达到100%的免疫合格率。对雏鸡也具有较好的免疫效果,免疫一次时H5和H7亚型抗体合格率分别在一免后3周和2周达到70%以上,并维持到一免后13周;进行二次免疫后,H5和H7亚型抗体合格率在二免后1周都达到100%。对雏鸭的免疫效果相对较差,一次免疫的H5亚型抗体水平不能达到70%的免疫合格标准,二免后1周H5和H7亚型抗体合格率均能达到80%以上,必须加强免疫一次才能达到满意的免疫效果。The new mutants of H7N9 avian influenza viruses have caused devastating poultry diseases and severe economic damage scince 2017. Here, we evaluated the immune effect of the inactivated H5 and H7 subtype avian influenza vaccine (H5N1 Re-8 and H7N9 Re-1) in chickens and ducks. According to the immune procedure made by Ministry of Agriculture of the People..s Republic of China, chickens and ducks were immunized with H5N1 Re-8 and H7N9 Re-1 vaccine and the hemagglutinin-inhibition (HI) antibody was detected on a weekly basis. Our results indicated that H5N1 Re-8 and H7N9 Re-1 vaccine induced strong immune response in egg-laying poultry, and 100% of participants in groups had high protective antibody levels in two weeks post one-time vaccination. For the first immunization in chickls, the H5 and H7 antibodies could reached 70% protective threshold in three and two weeks post one-time vaccination, respectively. Then, chicks inoculated for the second immunization had the 100% of H5 and H7 protective antibody levels in one week post two-time immunization. The H5 antibody level of ducklings vaccinated one time was bellow the 70% protective threshold, while the H5 and H7 antibody level of ducklings was above 80% in one week post two-time vaccination. Thus ducklings need two-time vaccination to get good immune response.

关 键 词：H7N9流感病毒 重组禽流感H5+H7二价灭活苗 免疫效果 

分 类 号：S852.4[农业科学—基础兽医学]