不同植物生长调节剂对复合盐胁迫下黑小麦种子萌发的影响  

Effects of Plant Growth Regulators on Germination of Black Wheat Seeds under Salt-stress

作　　者：王泽琼[1] 蔡珺[1] 韩国良 刘勇[2] WANG Ze-qiong;CAI Jun;HAN Guo-liang;LIU Yong(Wuhan Institute of Bioengineering,Wuhan,Hubei 430415,China;Institute of Fruit and Tea,Hubei Academy of Agricultural Science,Wuhan,Hubei 430064,China)

机构地区：[1]武汉生物工程学院,湖北武汉430415 [2]湖北省农业科学院果树茶叶研究所,湖北武汉430064

出　　处：《福建农业学报》2019年第3期358-363,共6页Fujian Journal of Agricultural Sciences

基　　金：农业部华中作物有害生物综合治理重点实验室开放课题(2018ZTSJJ9);湖北省自然科学基金项目(2017CFB163);武汉市产学研项目(CXY201643)

摘　　要：【目的】探讨α-萘乙酸钠(NAA)、赤霉素(GA3)和6-苄氨基嘌呤(6-BA)3种植物生长调节剂不同质量浓度对不同程度盐胁迫条件下黑小麦种子萌发的影响,为在盐渍地区种植黑小麦提供可能性和科学依据。【方法】以紫麦一号的种子为试验材料,采用纸床法分别测定不同质量浓度的3种植物生长调节剂对不同程度盐胁迫条件下黑小麦种子萌发的影响。【结果】在无盐胁迫条件下,NAA、GA3、6-BA质量浓度分别为150mg·L^-1、GA3200mg·L^-1和6-BA5mg·L^-1时,黑小麦种子的发芽率、发芽势等萌发指标均为最佳;复合盐(NaCl∶Na2SO4=1∶1)溶液浸种显著抑制了黑小麦种子的萌发;用不同质量浓度的植物生长调节剂预处理均可在一定程度上缓解复合盐胁迫对黑小麦种子萌发的抑制作用;在中低浓度(40~80mmol·L^-1)复合盐胁迫条件下,GA3200mg·L^-1和6-BA5mg·L^-1预处理均能有效缓解复合盐胁迫对种子萌发的影响;随着复合盐浓度的增加,各处理种子的发芽势和发芽率明显下降,当复合盐浓度为120mmol·L^-1时,只有6-BA5mg·L^-1预处理对缓解胁迫有一定作用。【结论】在中低复合盐浓度胁迫条件下,采用GA3(200mg·L^-1)或6-BA(5mg·L^-1)预处理能够缓解盐胁迫对黑小麦种子的伤害,从而显著促进在盐胁迫条件下黑小麦种子的萌发。【Objective】Effects of 3 plant growth regulators on the germination of black wheat seeds under salt-stress were studied to provide possible relief for the wheat planting in saline-alkali areas.【Method】 Using a paper bedding method,1-N aphthaleneacetic acid (NAA),gibberellic acid ( GA 3) or 6-benzylamino-purine (6-BA) was applied on seeds of black wheat (Zimai 1) to observe the germination under varied salt concentrations.【Result】The best rate,potential and other indices on the seed germination were found when 150 mg·L^-1 NAA,200 mg·L^-1 GA 3 or 5 mg·L^-1 6-BA was used without salt-stress. The presence of NaCl∶Na 2 SO 4 =1∶1 significantly inhibited the germination. Addition of the plant growth regulators in different concentrations relieved the inhibitive effect to a certain extent. Both GA 3 at 200 mg·L^-1 and 6-BA at 5 mg·L^-1 significantly alleviated the germination inhibition when medium to low salt-stress (i.e.,40-80 mmol·L^-1 ) was imposed on the seeds. The germination tendency and rate were significantly decreased with increasing salt-stress. When the salt concentration was raised to 120 mmol·L^-1 ,only the application of 6-BA 5 mg·L^-1 exhibited an effect on the germination.【Conclusion】 The treatment of GA 3 200 mg·L^-1 or 6-BA 5 mg·L^-1 could protect the black wheat seeds from being permanently harmed by a medium-to-low salt-stress and improve the germination.

关 键 词：黑小麦种子 萌发 植物生长调节剂 复合盐 胁迫 

分 类 号：S512.1[农业科学—作物学]