差速贴壁法在原代星形胶质细胞纯化中的应用  

Application of Velocity Dependent Adhesion Method in the Purification of Primary Astrocytes

作　　者：李艳[1] 张瑜 李斐雪 LI Yan;ZHANG Yu;LI Feixue(School of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 310036,China;School of Science,Hangzhou Normal University,Hangzhou 311121,China)

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036 [2]杭州师范大学理学院,浙江杭州311121

出　　处：《杭州师范大学学报（自然科学版）》2019年第3期268-273,共6页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：浙江省教育厅一般科研项目(Y201840060);杭州师范大学实验室开放项目(PD02011009007);杭州师范大学教改项目(4105C52119311070)

摘　　要：为获得高纯度的星形胶质细胞,在大鼠新生P0乳鼠大脑皮层的原代培养后,采用差速贴壁法予以纯化.经细胞免疫荧光染色鉴定,差速贴壁30min及1h处理获得的星形胶质细胞纯度高、状态好,能满足神经生物学研究中对高纯度星形胶质细胞培养的实验要求,适宜推广.High purity astrocytes were acquired after the primary cultures in P0 rat cortex tissues and the purification via velocity dependent adhesion method.By using immunofluorescence staining identification,the astrocytes with high purity obtained after adhering for 30~60 minutes,could satisfy the experimental requirements of neurobiology on the culture of astrocytes with high purity,they were suitable for popularization.

关 键 词：星形胶质细胞 差速贴壁法 大鼠 原代细胞培养 

分 类 号：Q28[生物学—细胞生物学]