紫花地丁不同提取物化学成分检识及抗炎活性研究  

Identification and anti-inflammatory activity of different extracts from viola yedoensis

作　　者：张静[1] 罗燕[1] 乔彦杰 梁小瑞 张保军 刘晓婷[1] 连科迅 杨莉[1] 谷新利[1] Zhang Jing;Luo Yan;Qiao Yanjie;Liang Xiaorui;Zhang Baojun;Liu Xiaoting;Lian Kexun;Yang Li;Gu Xinli(College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang 832003,China)

机构地区：[1]石河子大学动物科技学院

出　　处：《石河子大学学报（自然科学版）》2019年第1期121-126,共6页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(31460673);石河子大学高层次人才科研启动项目(RCSX201701)

摘　　要：目的研究紫花地丁乙醇回旋冷凝法分离乙酸乙酯提取物和水提取物的化学成分及抗炎活性。方法紫花地丁乙醇回旋冷凝法提取乙酸乙酯部位和水相部位,并对各部位进行化学成分检识。采用二甲苯诱导小鼠耳廓肿胀、小鼠腹腔毛细血管通透性、角叉菜胶诱导大鼠足趾肿胀进行急性炎症造模、炎性组织PGE2含量检测、NO含量检测等动物试验,研究紫花地丁不同提取物的抗炎作用。结果紫花地丁乙酸乙酯提取物中主要含有黄酮、蒽醌类,水提取物中主要含有糖及苷类、皂苷类。乙酸乙酯提物各剂量组对二甲苯致小鼠耳肿胀、角叉菜胶致小鼠足肿胀、小鼠腹腔毛细血管通透性均具有显著的抑制作用(P<0.05~P<0.01),并可不同程度降低炎性组织PGE2含量和NO含量,中剂量组作用极显著(P<0.01)。结论紫花地丁的抗炎作用活性成分在乙醇回旋冷凝法提取的乙酸乙酯提取物中;紫花地丁乙酸乙酯提取物抑制了炎性组织中PGE2的生成量和NO释放量,其抗炎作用可能与降低PGE2、NO表达有关。Objective To study the anti-inflammatory activity of ethyl acetate extract and water extract from Viola yedoensis by ethanol cyclotron condensation. Methods Ethyl acetate and water phase fractions were extracted by ethanol cyclotron condensation from Violet Flower Dibutylethanol,and the chemical constituents of each fraction were identified.In order to study the anti-inflammatory effects of different extracts of Radix Zihua Ding,xylene-induced ear swelling,abdominal capillary permeability,carrageenan-induced toe swelling in rats were used to establish acute inflammation model,detect the content of PGE2 in inflammatory tissues,and detect the content of NO. Results Flavonoids and anthraquinones are the main components of ethyl acetate extract of Viola florida,and sugar,glycosides and saponins are the main components of water extract.Ethyl acetate extract had significant inhibitory effects on xylene-induced ear swelling,carrageenan-induced foot swelling and abdominal capillary permeability in mice (P<0.05 - P <0.01),and could reduce the contents of PGE2 and NO in inflammatory tissues to varying degrees.The middle dose group had significant inhibitory effects(P<0.01). Conclusion Ethyl acetate extract of Viola Florida has significant anti-inflammatory effect,and its anti-inflammatory mechanism may be related to the decrease of PGE2 and NO expression.

关 键 词：紫花地丁 检识 化学成分 抗炎活性 

分 类 号：S853.74[农业科学—临床兽医学]