白茅内生菌Chaetomium globosum WQ来源的细胞松弛素类化合物研究  

Research of cytochalasan compounds from endophyte Chaetomium globosum WQ in Imperata cylindrical

作　　者：陈陆敏 朱力 刘琴[3] 陈倩倩 袁丹 申丽 CHEN Lumin;ZHU Li;LIU Qin;CHEN Qianqian;YUAN Dan;SHEN Li(College of Medicine (Institute of Translational Medicine),Yangzhou University,Yangzhou 225009,China;Jiangsu Key Labrratrry of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases,Yangzhou,225009,China;Jiangsu Lixiahe Institute of Agricultural Science,Yangzhou 225007,China)

机构地区：[1]扬州大学医学院(转化医学研究院),江苏扬州225009 [2]江苏省中西医结合老年病防治重点实验室,江苏扬州225009 [3]江苏里下河地区农业科学研究所,江苏扬州225007

出　　处：《扬州大学学报（农业与生命科学版）》2019年第3期101-106,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏省高等学校自然科学研究重大项目(17KJA350005);江苏省重点研究计划(现代农业)项目(BE2017339);扬州市重点研发计划项目(YZ2017043、YZ2018063);扬州大学研究生实践创新计划项目(XSJCX17-039);扬州大学大学生科技创新基金项目(X20170851)

摘　　要：为研究白茅内生菌Chaetomium globosum WQ固体发酵产物的化学成分,以1H-NMR谱和TLC为指导,采用硅胶柱层析、Sephadex LH-20凝胶柱层析、反相柱层析和高效液相色谱等方法对C.globosum WQ固体发酵产物乙酸乙酯相的化学成分进行分离纯化,并采用MTT法测定化合物对氧糖剥夺(OGD)模型大鼠星形胶质细胞的体外神经保护活性。结果表明:共分离获得5个细胞松弛素类化合物cytoglobosin Ab(1)、chaetogloKosin W(2)、chaetoglobosin V(3)、chaetoglobosin Vb(4)和cytoglobosin I(5);体外活性测定发现,cytoglobosin Ab(1)能明显降低OGD引起的大鼠星形胶质细胞死亡,且呈浓度依赖性。这一研究提示cytoglobosin Ab(1)可能是治疗新生儿缺氧缺血性脑病(NHIE)潜在的神经保护剂。To investigate the chemical constituents from the solid culture of endophyte Chaetomium globosum WQ associated with Imperata cylindrical,guided by 1H-NMR spectrum and TLC,isolation and purification of the ethyl acetate extract of the solid culture of C.globosum WQ was carried out through silica gel column chromatography,gel filtration over Sephadex LH-20,ODS column chromatography and HPLC.Then,in vitro neuroprotective effect of compounds a-gainst oxygen glucose deprivation(OGD)-induced damage in rat astrocytes was evaluated by MTT method.Five cytochalasan compounds cytoglobosin Ab(1),chaetoglobosin W(2),chaetoglobosin V(3),chaetoglobosin Vb(4)and cytoglo-bosin I(5)were obtained.In vitro neuroprotective effect assay showed that cytoglobosin Ab(1)could attenuate OGD induced cell death in rat astrocytes in a dose-dependent manner obviously,which indicated that cytoglobosin Ab(1)would be a potential neuroprotective agent in treatment of Newborn Hypoxic Ischemic Encephalopathy(NHIE).

关 键 词：CHAETOMIUM globosum 细胞松弛素类化合物 神经保护活性 氧糖剥夺 大鼠星形胶质细胞 

分 类 号：R284.2[医药卫生—中药学]