大鼠膈神经、肋间神经侧侧缝合对肺功能的影响  

Effects of side-to-side neurorrhaphy of phrenic nerve or intercostal nerve on pulmonary function in rats

作　　者：芮晶 许娅莉 赵新 李继峰 劳杰 顾玉东 RuiJing;Xu Yali;Zhao Xin;Li Jifeng;Lao Jie;Gu Hidong(Department of Hand Surgery, Huashan Hospital, Key Laboratory of Hand Reconstruction of Health Ministry, Shanghai Key Laboratory ofPeripheral Nerve and Microsurgery, Shanghai200040, China)

机构地区：[1]复旦大学附属华山医院手外科,卫生部手功能重建重点实验室,上海市周围神经显微外科重点实验室,上海200040

出　　处：《中华手外科杂志》2019年第3期212-215,共4页Chinese Journal of Hand Surgery

基　　金：国家自然科学基金青年项目(81501051);国家自然科学基金面上项目(81572127).

摘　　要：目的验证大鼠膈神经、肋间神经通过自体神经移植侧侧缝合至切断再缝合的肌皮神经缝合口的手术安全性。方法取SD大鼠54只,随机分成三组,每组18只:阴性对照组(A组)、膈神经组(B组)、肋间神经组(C组)。A组为肌皮神经切断直接缝合,另两组通过腓肠神经移植分别将膈神经及肋间神经侧侧缝合至修复过的肌皮神经缝合口。于术后1、2、3个月进行肺功能测定。结果三组大鼠术后1、2、3个月各指标,包括呼吸频率(F)、分钟通气量(MV)、吸气时间(Ti)、呼气时间(Te)、吸气峰值流速(PIF)、呼气峰值流速(PEF)和50%容量呼气流速(EF50),比较结果差异均无统计学意义(P>0.05)。结论侧侧缝合的手术方式对肺功能无明显损伤,这种新的手术方式安全,在临床应用上有较大优势。Objective To verify the safety of a new surgical model by assessing the lung fiinction of phrenic nerve and intercostal nerve via side-to-side with nerve autograft to the neurorrhaphy site of the musculocutaneous nerve in rats. Methods Fifty four SD rats were randomly divided into three groups. Group A was tiie negative control group, in which the musculocutaneous nerve was transected and directly sutured. The o&er two groups were the phrenic nerve (group B) and intercostal nerve (group C): after the musculocutaneous nerve was transected and the side-to-side neuronhaphy was conducted. Sural nerve was used to connect the musculocutaneous nerve neurorrbaplty site and the donor nerve as phrenic nerve or intercostal nerve. Lung function test was conducted at 1,2 and 3 months postoperatively Results No significant difference was detected from each parameter including breathing frequency (F)s minute ventilation (MV), inspirationand expiration time (Ti and Te);peak inspiratory and expiratoiy flow (PIF and PEF) and 50% expiratory flow (EF50) oflung function test among group A, B and Cat 1,2 and 3 months postoperatively. Conclusion Side-to-side neurorrhaphy has no obvious damage to pulmonaiy function. This new method is safe and has great advantages in clinical application.

关 键 词：大鼠 呼吸功能试验 膈神经 肋间神经 神经侧侧缝合 

分 类 号：R688[医药卫生—骨科学]