紫地榆提取物对慢性溃疡性结肠炎大鼠的治疗作用  

The Effect of Extracts from Geranium strictipes R. Knuth on the Chronic Ulcerative Colitis in Rats

作　　者：耿玲 杨国堂 张旭强 秦菁莉 陈俊雅 李辉 GENG Ling;YANG Guotang;ZHANG Xuqiang;QIN Jingli;CHEN Junya;LI Hui(College of Medicine,Dali University,Dali 671000 Yunnan,China;College of Pharmaceutical Sciences,Dali University,Dali 671000 Yunnan,China)

机构地区：[1]大理大学基础医学院,云南大理671000 [2]大理大学药学与化学学院,云南大理671000

出　　处：《中药新药与临床药理》2019年第6期653-658,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金项目(30860365);大理大学创新训练基金项目(KYXL2018-08)

摘　　要：目的考察紫地榆乙酸乙酯提取物(EAEG)对慢性溃疡性结肠炎(UC)大鼠的治疗作用。方法采用免疫造模法制备UC大鼠模型。将大鼠随机分为空白组,模型组,柳氮磺胺吡啶组(SASP,100mg·kg^-1),EAEG低、中、高剂量组(50、100、200mg·kg^-1),灌胃给药(10mL·kg^-1),每日1次,连续给药15d。观察大鼠一般情况,并测定大鼠体质量指数、结肠湿质量指数及胸腺、脾脏、肝脏脏器指数;HE染色观察结肠、脾脏组织病理学变化;采用分光光度法测定结肠组织和血清中丙二醛(MDA)、超氧化物歧化酶(SOD)及髓过氧化物酶(MPO)活性;采用双抗体夹心ELISA法测定血清中肿瘤坏死因子α(TNF-α)、白介素6(IL-6)含量。结果与空白组比较,模型组大鼠的体质量指数、胸腺指数显著降低(P<0.01),结肠湿质量指数、脾脏指数及肝脏指数显著升高(P<0.01),血清中MDA、MPO、TNF-α、IL-6含量明显升高(P<0.01),SOD活性明显降低(P<0.01),结肠组织中MDA含量明显增加(P<0.01)。与模型组比较,EAEG高、低剂量组的体质量指数明显升高(P<0.05);EAEG中、低剂量组的结肠湿质量指数明显降低(P<0.05);EAEG各剂量组的脾脏指数均明显降低(P<0.05,P<0.01),EAEG高剂量组的肝脏指数明显降低(P<0.05),而对胸腺指数的影响不明显(P>0.05);EAEG高、中剂量组的结肠组织中MDA含量、血清中TNF-α含量明显降低(P<0.05,P<0.01);EAEG各剂量组的血清MPO、IL-6含量均明显降低(P<0.05,P<0.01);EAEG高剂量组血清中SOD活性明显增强(P<0.05)。模型组结肠黏膜有大量炎性细胞浸润,局部可见黏膜层完全脱落,出现组织坏死,EAEG高、中剂量组观察到局部肉芽组织的修复。结论EAEG对UC大鼠有一定的治疗作用,可能与其提高机体抗氧化能力和降低TNF-α、IL-6等炎症因子表达有关。Objective To investigate the effect of the ethyl acetate extracts from Geranium strictipes R.Knuth(EAEG)on the chronic ulcerative colitis(UC)in rats.Methods The ulcerative colitis rat models were established using immunological method.The animals were randomly divided into five groups:model group,low,middle and high dosage(50,100,200 mg·kg^-1)of EAEG and the sulfasalazine(SASP)group(100 mg·kg^-1),which were for i.g.15 days continuously.The change of general conditions of the rats is observed,the weight,colon,thymus,spleen and liver indexes were measured,and the pathological change of colon and spleen in rats were observed at the end of the experiment.Meanwhile,the content of malondialdehyde(MDA),the activities of myeloperoxidase(MPO)and superoxide dismutase(SOD)and in the colon tissues and serum were measured by spectrophotometry.In addition,the contents of the TNF-α,IL-6 in serum of rats were examined by ELISA.Results The weight indexes of rats in model group,SASP group and EAEG groups were declined(P<0.01)compared with blank group.Compared with model group,the weight indexes of rats in EAEG high and low dose groups increased(P<0.05);the colon indexes of rats in EAEG medium and low dose groups decreased(P<0.05);the spleen indexes of rats in all EAEG groups decreased(P<0.05;P<0.01);and the liver index of rats in high dose EAEG group decreased(P<0.05)with no obvious effect on thymus index(P>0.05).The serum contents of MDA and TNF-a in rats of high and medium dose EAEG groups decreased(P<0.05,P<0.01).The serum contents of MPO and IL-6 in rats of all EAEG groups decreased(P<0.05,P<0.01),the MPO activities and contents of MDA of rats in all EAEG groups decreased(P<0.05;P<0.01).The activity of SOD in serum of rats in high dose EAEG group was enhanced(P<0.05).In the model group the inflammatory cell infiltration,thorough colonic mucous membrane abscission,tissue mortification and focal ulceration appeared in the colon tissue;however,the local repair of granulation tissue for llie damnification was observed in the hi

关 键 词：紫地榆 乙酸乙酯提取物 慢性溃疡性结肠炎 超氧化物歧化酶 丙二醛 肿瘤坏死因子Α 白介素6 

分 类 号：R285.5[医药卫生—中药学]