风湿性心脏瓣膜病合并心房颤动患者的DNMT3A表达与Hcy水平  

DNMT3A expression and Hcy level in patients with rheumatic valvular disease complicated with atrial fibrillation

作　　者：倪世豪 代晨[1,2] 秦润禾 陶辉[1,2] 石开虎[1,2] NI Shi-hao;DAI Chen;QIN Run-he;TAO Hui;SHI Kai-hu(Department of Cardiothoracic Surgery,The Second Affiliated Hospital of Anhui Medical University,Hefei,Anhui 230601,China)

机构地区：[1]安徽医科大学第二附属医院心胸外科,安徽合肥230601 [2]安徽医科大学心血管病研究中心,安徽合肥230601

出　　处：《中国临床研究》2019年第5期593-596,共4页Chinese Journal of Clinical Research

基　　金：国家自然科学基金(81570295);安徽省科技攻关计划项目(1501041148)~~

摘　　要：目的探讨风湿性心脏瓣膜病(风心病)合并心房颤动(AF)患者心肌组织中DNA甲基转移酶3A(DNMT3A)的表达与血清同型半胱氨酸(Hcy)水平。方法以2016年9月至2017年10月22例风心病接受心脏瓣膜置换手术患者作为研究对象,按照是否合并AF分为AF组(n=11)和窦性心律(SR)组(n=11)。分析比较两组患者心功能各项指标;分别应用Westernblot法和免疫组化法检测患者术中切取的右心耳心肌组织中DNMT3A蛋白表达水平;ELISA法检测患者血清中Hcy的含量。结果Westernblot结果显示,AF组DNMT3A在心肌组织中的表达明显高于SR组(P<0.01)。AF组中Hcy含量显著高于SR组,差异有统计学意义(P<0.01)。AF与SR免疫组化结果比较,AF患者心肌重构明显,DNMT3A在AF组中的阳性表达为9例,在SR组中的阳性表达为3例,两组比较差异有统计学意义(P<0.05)。DNMT3A蛋白与Hcy在风心病合并AF患者体内的表达呈正相关(r=0.573,P<0.01)。结论心肌组织中DNMT3A表达与血清Hcy水平在风心病合并AF患者中均增加,可能共同参与风心病合并AF的发生发展。Objective To investigate the expression of DNA methyltransferase 3A ( DNMT3A) in myocardium and serum homocysteine ( Hcy) levels in patients with rheumatic valvular heart disease ( RHD) complicated with atrial fibrillation ( AF) underwent.Methods Total 22 patients with rheumatic heart disease heart valve replacement surgery from September 2016 to October 2017 were taken as the research objects and were divided into AF group ( n = 11) and sinus rhythm( SR) group ( n = 11).The level of DNMT3A in myocardial tissue were detected by Western Blotting method and immunohistochemical method;serum Hcy level was detected by enzyme-linked immunosorbent assay ( ELISA).The indexes were analyzed and compared between two groups.Results Western blot showed that the expression of DNMT3A increased in AF group compared with SR group ( P < 0.01);immunohistochemical results showed that the positive expression rates of DNMT3A increased significantly in AF group compared with SR group;ELISA detection showed that serum Hcy content in AF group was significantly higher than that in SR group ( P < 0.01 ).The immunohistochemistry results showed that myocardial remodeling was obvious in AF patients.The positive expression of DNMT3A was found in 9 cases in AF group and 3 cases in SR group, and there was significant difference between two groups ( P < 0.05).The correlation analysis showed that there was a positive correlation between DNMT3A and Hcy ( r = 0.573,P < 0.01).Conclusion The increase of DNMT3A expression and Hcy level in RHD patients complicated with AF may participate in the development of rheumatic heart disease with AF.

关 键 词：风湿性心脏瓣膜病 心房颤动 DNA甲基转移酶3A 同型半胱氨酸 

分 类 号：R541.2[医药卫生—心血管疾病] R541.75[医药卫生—内科学]