蓝莓根系对土壤锰胁迫的生理响应  

Physiological response of blueberry roots to Mn^(2+) stress in soil

作　　者：董珊珊[1] 李宁冉 杨海燕[1] 吴文龙[1] 闾连飞[1] 李维林[1,3] DONG Shanshan;LI Ningran;YANG Haiyan;WU Wenlong;LU Lianfei;LI Weilin(Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China;Co-Inn ovationCenter for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University,Nanjing 210037, China;Ninghai Middle School, Nanjing 210036, China)

机构地区：[1]江苏省中国科学院植物研究所,江苏南京210014 [2]宁海中学,江苏南京210036 [3]南京林业大学南方现代林业协同创新中心南京林业大学林学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2019年第3期169-174,共6页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：南京市科技计划项目(201716052);江苏省农业科技自主创新资金项目[CX(17)2011];江苏现代农业产业技术体系项目(JATS[2018]317)

摘　　要：【目的】了解蓝莓对土壤中锰元素的耐受能力,为蓝莓的高效栽培和安全生产提供依据。【方法】盆栽条件下采用不同浓度(0、1.0、2.5、5.0、10.0 mmol/L)锰离子(Mn^(2+))浇灌处理蓝莓品种‘Gardenblue’和‘Tifblue’,测定根系超氧阴离子■产生速率,以及过氧化氢(H_2O_2)、丙二醛(MDA)、可溶性蛋白(SP)、抗坏血酸(AsA)和还原型谷胱甘肽(GSH)含量变化,通过分析蓝莓根系的生理反应,评估蓝莓对土壤锰的耐受能力。【结果】土壤外源Mn^(2+)处理浓度低于2.5 mmol/L对植株的正常生长无影响,当处理浓度达到或超过2.5 mmol/L时对植株正常生长产生一定的影响。随着Mn^(2+)浓度增加,蓝莓根系中■产生速率和H_2O_2、MDA含量均在2.5 mmol/L Mn^(2+)处理后变化显著,SP、AsA和GSH含量亦变化显著,其中‘Gardenblue’各指标的变化较‘Tifblue’平缓。分析认为蓝莓对土壤锰胁迫具有较强的耐受性,其耐受机理主要是由于植株体内抗氧化物质如SP、AsA和GSH等的增加。【结论】蓝莓植株内存在多种调控锰胁迫的机制,其对土壤锰胁迫具有较强的耐受性,耐受阈值为2.5 mmol/L。【Objective】 This test was carried out to understand the tolerance of blueberry to Mn stress in soil in order to provide basic references for blueberry cultivation.【Method】 Potted blueberry cultivars ‘Gardenblue’ and‘Tifblue’ were irrigated with 0, 1.0, 2.5, 5.0, 10.0 mmol/L Mn^2+, and six physiological indicators including the generation rate of O2, content of hydrogen peroxide(H2O2), malondialdehyde(MDA), soluble protein(SP), ascorbic acid(AsA) and glutathione(GSH) in root were determined, the physiological response and tolerance to Mn^2+ stress were evaluated.【Result】Less than 2.5 mmol/L Mn^2+ added in soil was innocuous for the growth of blueberry, over 2.5 mmol/L Mn^2+ added in soil had negative effect on the growth of blueberry. With the increase of Mn^2+ concentration, significant changes of O2 generation rate, H2O2 and MDA content, as well as SP, AsA and GSH content in roots appeared at 2.5 mmol/L and more concentrations, of which, these changes of ‘Gardenblue’ were slighter than ‘Tifblue’. It is inferred blueberry has strong tolerance to Mn stress in soil, and the tolerance is based on the alterations of antioxidants such as SP, AsA and GSH.【Conclusion】Blueberry has a regulating mechanism for Mn stress, which lead to the strong tolerance to Mn with a threshold value of 2.5 mmol/L Mn^2+ concentration in soil.

关 键 词：蓝莓 锰胁迫 生理响应 

分 类 号：S663.2[农业科学—果树学]