俄罗斯桃叶卫矛组织培养苗对盐胁迫的生理响应  

Physiological response of Euonymus bungeanus tissue culture seedlings to salt stress

作　　者：支梦玲 金庆东 华雨 马艳 Zhi Mengling;Jin Qingdong;Hua Yu;Ma Yan(College of Horticulture,Jinling Institute of Technology,Nanjing 210000,China)

机构地区：[1]金陵科技学院园艺园林学院

出　　处：《江苏林业科技》2019年第2期1-6,共6页Journal of Jiangsu Forestry Science & Technology

基　　金：江苏省自然基金项目(BK200151097);金陵科技学院高层次人才引进资助项目(jit-rcyj-201507);江苏省高校大学生创业创新训练计划项目“盐胁迫对桃叶卫矛组培苗生长和生理特性的研究”(201713573058X)

摘　　要：采用盆栽方法,以俄罗斯桃叶卫矛组织培养苗为材料,用不同浓度(0,40,80,120mmol/L)的NaCl溶液进行处理,对盐胁迫下俄罗斯桃叶卫矛的形态表现和叶绿素含量、膜透性、丙二醛(MDA)、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性等生理指标进行研究。结果表明:在盐胁迫下植物的株高生长量逐渐受到抑制;叶绿素含量随盐浓度的增加呈现下降趋势;膜透性、MDA、SOD、POD活性均随盐浓度增加而增加;40—80mmol/L的NaCl低浓度溶液胁迫后促进了俄罗斯桃叶卫矛根冠比的增加,而高浓度(120mmol/L)NaCl溶液抑制了根冠比的增加。进一步酶活指标分析表明,在盐胁迫下,植株的叶片质膜结构受到一定的伤害,但MDA含量差异不明显,而SOD和POD活性随着盐浓度升高显著上升。研究认为,俄罗斯桃叶卫矛通过提高植株的抗氧化能力来抵抗盐胁迫造成的伤害,对盐胁迫具有较强的适应能力。In this study,the physiological response of Euonymus bungeanus tissue culture seedlings under different concentrations of NaCl stress was studied by potted culture.The results showed that the growth of plant height was inhibited under salt stress,and the chlorophyll content of leaves decreased with the increase of salt concentration;membrane permeability,malondialdehyde(MDA),SOD,and POD activities were all increased with the increase of salt concentration.The low concentration of 40-80 mmol/L NaCl promoted the increase of the root-shoot ratio while high concentration of 120 mmol/L NaCl inhibited it.Furthermore,the enzyme activity index analysis showed that the leaves suffered from a certain degree of damage under salt treatment without significant changes of MDA content while SOD and POD enzyme activities increased significantly,suggesting that E.bungeanus could get tolerance to salt stress by improving the antioxidant systems.

关 键 词：俄罗斯桃叶卫矛 耐盐性 NACL 胁迫 生理指标 组织培养苗 

分 类 号：Q945.78[生物学—植物学] S792.99[农业科学—林木遗传育种]