楸叶泡桐杂种无性系9503的组织培养技术研究  

Research of tissue culture in hybrid clone 9503 of Paulownia catalpifolia

作　　者：王红玲[1] 孙倩 黄瑞芳[1] Wang Hongling;Sun Qian;Huang Ruifang(Jiangsu Academy of Forestry,Nanjing 211153,China;Nantong Vocational College of Science & Technology,Nantong 226007,China)

机构地区：[1]江苏省林业科学研究院,江苏南京211153 [2]南通农业职业技术学院,江苏南通226007

出　　处：《江苏林业科技》2019年第2期43-45,52,共4页Journal of Jiangsu Forestry Science & Technology

基　　金：江苏省科技支撑(现代农业)项目“适于平原林网更新的泡桐和落羽杉优质材新品种选育”(BE2015371);中央财政林业科技推广示范资金项目“苏北杨树更新良种繁育及高效培育技术推广”(苏[2017]TG03号)

摘　　要：以楸叶泡桐和白花泡桐杂交无性系的枝条为外植体,开展了离体芽增殖技术,以及愈伤组织诱导、芽分化和不定芽生根等组织培养技术的研究。结果表明,增殖培养基采用MS+8mg/L6-BA+0.3mg/LNAA,不定芽的增殖倍数可达到9;利用其叶片进行愈伤组织诱导的最佳培养基为MS+12mg/L6-BA+0.4mg/LNAA,诱导率高达95%;愈伤组织分化的最佳培养基为MS+10mg/L6-BA+0.7mg/LNAA,不定芽生根的最佳培养基为1/2MS+0.5mg/LIBA+0.3mg/LNAA,生根率100%。By using excellent stem segments of the hybrid Clone 9503 of Paulownia fortunei and P.catalpifolia as explants,we studied the tissue culture technology,including callus induction,bud differentiation and bud proliferation.The results showed that the multiplication medium was MS+8 mg/L 6-BA+0.3 mg/L NAA,with 9 of multiplication time.The suitable medium for leaf callus induction was MS+12 mg/L 6-BA+0.4 mg/L NAA,with the induction rate of 95%.The suitable callus differentiation medium was MS+10 mg/L 6-BA+0.7 mg/L NAA.The suitable rooting culture medium was 1/2MS+0.5 mg/L IBA+0.3 mg/L NAA,with the rooting rate of 100%.

关 键 词：愈伤组织 外植体 增殖 楸叶泡桐 

分 类 号：Q943.1[生物学—植物学] S792.43[农业科学—林木遗传育种]