健脾益气方剂含药血清对胃癌细胞生长及血管新生的影响  

Effect of Jianpi Yiqi Prescription serum on the gastric cancer cell growth and angiogenesis

作　　者：王兴龙 WANG Xing-long(Department of Pharmacy, Beijing Tongren Hospital, Beijing, 100176, China)

机构地区：[1]北京同仁医院药学部,北京100176

出　　处：《海南医学院学报》2019年第10期750-753,757,共5页Journal of Hainan Medical University

基　　金：2014年度首都医科大学附属北京同仁医院科研基金项目(2014-YJJ-ZZL-020)~~

摘　　要：目的:研究健脾益气方剂含药血清对胃癌细胞生长及血管新生的影响。方法:选择SD大鼠,分别给予健脾益气药液及生理盐水灌胃后取主动脉血,制备健脾益气方剂含药血清及空白血清;培养胃癌SGC-7901细胞,分为10%含药血清组、10%空白血清组及10%胎牛血清组,处理24 h测定细胞增殖活力、凋亡指数以及增殖基因、凋亡基因、血管新生基因的表达量。结果:10%含药血清组的细胞增殖活力值以及细胞中EZH2、UHRF1、CyclinA、CyclinB1、CDK1、c-IAP1、XIAP、VEGF、VEGFR2、HIF-1α、COX2的mRNA表达量明显低于10%空白血清组及10%胎牛血清组,凋亡指数以及细胞中Caspase-3、PDCD5的mRNA表达量明显高于10%空白血清组及10%胎牛血清组。结论:健脾益气方剂含药血清对胃癌细胞生长及血管新生均具有抑制作用。Objective: To study the effect of Jianpi Yiqi Prescription serum on the gastric cancer cell growth and angiogenesis. Methods: SD rats were selected and given intragastric administration of Jianpi Yiqi solution and normal saline respectively to collect aorta blood and prepare the Jianpi Yiqi Prescription serum and blank serum;gastric cancer SGC-7901 cells were cultured and divided into 10% drug serum group, 10% blank serum group and 10% fetal bovine serum group, and the cell proliferation activity, apoptosis index as well as proliferation gene, apoptosis gene and angiogenesis gene expression were measured after 24 h of treatment. Results: Cell proliferation activity as well as EZH2, UHRF1, CyclinA, CyclinB1, CDK1, c-IAP1, XIAP, VEGF, VEGFR2, HIF-1α and COX2 mRNA expression in cells of 10% drug serum group was significantly lower than those of 10% blank serum group and 10% fetal bovine serum group while apoptosis index as well as Caspase-3 and PDCD5 mRNA expression in cells was significantly higher than those of 10% blank serum group and 10% fetal bovine serum group. Conclusion: Jianpi Yiqi Prescription serum can inhibit the gastric cancer cell growth and angiogenesis.

关 键 词：胃癌 健脾益气方 增殖 凋亡 血管新生 

分 类 号：R285.5[医药卫生—中药学]