Klotho对滋养细胞侵袭的影响  

Effect of Klotho on HTR-8/SVneo cells invasion

作　　者：薛筱蕾 成芳[1] 范翠芳[2] 雷帝 胥保梅[1] 蒋惠玲[1] XUE Xiao-lei;CHENG Fang;FAN Cui-fang;LEI Di;XU Bao-mei;JIANG Hui-ling(Department of Obstetrics, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, China;Department of Obstetrics, Wuhan University People's Hospital, Wuhan, Hubei 430060, China)

机构地区：[1]新疆医科大学第五附属医院产科,新疆乌鲁木齐830000 [2]武汉大学人民医院产科,湖北武汉430060

出　　处：《海南医学院学报》2019年第10期754-757,共4页Journal of Hainan Medical University

基　　金：乌鲁木齐市科学技术计划项目(Y131310002)~~

摘　　要：目的:研究Klotho对绒膜外滋养细胞系HTR8/SVneo侵袭的影响。方法:用免疫组化检测子痫前期及正常孕妇胎盘组织Klotho蛋白表达情况。实验分组为对照组、Klotho重组蛋白组、Klotho siRNA组。Transwell小室法检测侵袭能力,Western Blot检测细胞蛋白表达水平。结果:子痫前期胎盘组织Klotho表达水平明显低于正常胎盘组织。Klotho重组蛋白处理细胞后,HTR8/SVneo细胞侵袭能力显著增强、其表达的基质金属蛋白酶2(MMP-2)及基质金属蛋白酶9(MMP-9)表达上调。Klotho siRNA处理细胞后,HTR8/SVneo细胞侵袭能力显著减弱、其表达的MMP-2及MMP-9表达显著下调。结论:Klotho可能通过介导MMP-2和MMP-9的表达调节滋养细胞侵袭能力,为子痫前期发病机制的研究提供了新线索。Objective: To investigate the effect of Klotho on invasion of Extrachorionic trophoblastic cell line HTR-8/SVneo cells. Methods: Klotho protein expression in placenta of preeclampsia and normal pregnant women was detected by immunohistochemistry. Experimental grouping: Normal control group, Klotho recombinant protein group, Klotho SiRNA group. The abilities of cell were measured by Transwell assay. The protein expression were detected by Western blot. Results: The expression of Klotho in placenta of preeclampsia was significantly lower than that in normal placenta. Treatment with Klotho recombinant protein improved the HTR-8/SVneo cell invasion abilities, and upregulated the expression of MMP-2 and MMP-9. The effects were re-versed by treatment with Klotho siRNA. Conclusion: Klotho regulate the invasion of trophoblast may via MMP-2 and MMP-9 expression. It provides a new clue for the study of the pathogenesis of preeclampsia.

关 键 词：子痫前期 KLOTHO 细胞侵袭 HTR-8/SVneo细胞 

分 类 号：R714.244[医药卫生—妇产科学]