C、D型貂源铜绿假单胞菌的生物学特性鉴定及免疫效果评价  

Identification of Biological Characteristics and Evaluation of Immune Effect of Pseudomonas Aeruginosa C and D Strains from Mink

作　　者：吕娇 闫明灏 韩坤[3] 程悦宁[3] 廉士珍[3] 徐淑娟[3] 史秋梅[4] 陈丽凤[4] 曲学忠 丛培强 赵辉 闫喜军[3] 白雪[3] LV Jiao;YAN Ming-hao;HAN Kun;CHENG Yue-ning;LIAN Shi-zhen;XU Shu-juan;SHI Qiu-mei;CHEN Li-feng;QU Xue-zhong;CONG Pei-qiang;ZHAO Hui;YAN Xi-jun;BAI Xue(Jilin Agricultural University,Changchun,Jilin,130118,China;Lanzhou Veterinary Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu,730000,China;Institute of Special Animal and Plant Science of CAAS,Changchun,Jilin,130122,China;Institute of Animal Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei,066004,China;Animal Husbandry and Veterinary Technology Service Center,Wendeng,Shandong,264400,China;Dunhua Animal Husbandry Bureau,Yanbian Korean Autonomous Prefecture,Yanbian,Jilin,133700,China)

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]中国农业科学院兰州兽医研究所,甘肃兰州730000 [3]中国农业科学院特产研究所,吉林长春130000 [4]河北科技师范学院动物科技学院,河北秦皇岛066004 [5]山东省威海市文登区畜牧兽医技术服务中心,山东文登264400 [6]敦化市畜牧局,吉林省延边朝鲜族自治州,吉林延边133700

出　　处：《动物医学进展》2019年第6期23-29,共7页Progress In Veterinary Medicine

基　　金：国家自然科学基金青年科学基金(31502102);吉林省科技发展计划项目(20190401006NY)

摘　　要：从辽宁和黑龙江2个水貂养殖场死亡水貂肺中分离出13株铜绿假单胞菌,血清型鉴定分别为C型和D型,命名为PK13-C和H2F-D。为了解其作为疫苗株的免疫效果,测定了其运动能力、绿脓菌素生成能力、生长曲线、毒力、免疫原性及耐药性。结果表明,PK13-C对小鼠和水貂的毒力分别为7.5×106CFU和7×105CFU,泳动能力及群集运动能力较强,抽动能力较弱,生物被膜形成能力中等;H2F-D对小鼠和水貂的毒力分别为2.05×107CFU和2.2×106CFU,泳动能力较弱,但群集运动及抽动能力较强,生物被膜形成能力较弱。二者对本血清型菌株的免疫保护率均为100%,对异种血清型菌株的保护率为80%~90%。2个株菌均可产生绿脓菌素。生长曲线显示2个菌株于4h进入对数生长期,于18h左右进入平稳期。药敏试验结果表明,2个株菌对氯霉素、复方新诺明、氨苄西林/舒巴坦耐药。We isolated 13 Pseudomonas aeruginosa(PA)strains from the lungs of mink at two mink farms in Dalian and Heilongjiang.The serotypes were identified as C and D.We named them as PK13-C and H2F-D respectively.In order to determine the immune effect of the two strains as the vaccine strain,we measured its moving ability,pyocyanin,growth curve,immunogenicity and drug resistance.The results showed that the LD50 of PK13-C to mice was 7.5×10 6 CFU and to mink was 7×10 5 CFU,the swimming ability and the swarming ability were strong,the twitching ability was weak.It can produce the pyocyanin and the biofilm formation ability was medium.The LD 50 of H2F-D to mice was 2.05×10 7 CFU and to mink was 2.2×10 6 CFU,the swimming ability was weak,but the swarming ability and the twitching ability were strong,and it could also produce the pyocyanin and the ability of biofilm formation was weak.The immunological protection rates for PK13-C and H2F-D to homologous serotypes were both 100%,the protection rate for the heterologous serotypes was 80%-90%.The growth curve showed that the two strains entered logarithmic growth phase at 4 h and entered a stationary phase at 18 h.The results of drug sensitivity test showed the resistance to CAP,SXT and SAM.

关 键 词：铜绿假单胞菌 C型 D型 免疫原性 

分 类 号：S852.6[农业科学—基础兽医学]