丹艾痛经贴的提取工艺研究  

Study on Extraction Process of Danai Dysmenorrhea Paste

作　　者：宋军帅 张文峰 甘兴华 王欣 魏永利[3] 李克明[4] SONG Jun-shuai;ZHANG Wen-feng;GAN Xing-hua;WANG xin;WEI Yong-li;LI Ke-ming(Rushan People’s Hospital,Rushan 264500,China;Yuncheng County Hospital of Traditional Chinese Medicine,Yuncheng 274700,China;The Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China;Shandong Academy of Chinese Medicine,Jinan 250014,China)

机构地区：[1]乳山市人民医院,山东乳山264500 [2]郓城县中医医院,山东郓城274700 [3]山东中医药大学附属医院,山东济南250014 [4]山东省中医药研究院,山东济南250014

出　　处：《食品与药品》2019年第3期191-194,共4页Food and Drug

基　　金：山东省自然科学基金项目(编号ZR2016HL52)

摘　　要：目的建立丹艾痛经贴的最佳提取工艺。方法采用超临界CO2提取法提取肉桂、香附、艾叶挥发油,正交试验筛选最优提取工艺;采用高效液相法测定醇提药材中丹参酮ⅡA与丹酚酸B含量,以二者为指标进行正交试验优选最佳工艺。结果优选肉桂最佳提取条件为提取压力35MPa、提取温度40℃、CO2流量30kg/h。香附最佳工艺条件为温度45℃、提取压力30MPa、CO2流量5.5kg/h。艾叶最佳工艺条件为提取压力为20MPa,提取温度为30℃,CO2流量24kg/h;正交试验优选最佳醇提条件为10倍量75%乙醇,回流提取3次,每次1.5h。结论本实验优选提取工艺简便,重复性好且准确可靠,可为中药贴剂的制备提供参考。Objective To establish the optimal extraction process of Danai Dysmenorrhea Paste.Methods The volatile oil was extracted from Cinnamomi cortex,Cyperi rhizoma and Artemisiae argyi folium by supercritical CO2 extraction.The optimal extraction process was screened by an orthogonal experiment with the contents of tanshinoneⅡA and salvianolic acid B in alcohol extract determined by HPLC as indexes.Results The optimal extraction conditions for Cinnamomi cortex were the extraction pressure of 35 MP,extraction temperature of 40℃and CO2 flow rate of 30 kg/h.The optimal extraction conditions for Cyperi rhizoma were the extraction pressure of 30 MPa,extraction temperature of 45℃and CO2 flow rate of 5.5 kg/h.The optimal extraction conditions for Artemisiae argyi folium were the extraction pressure of 20 MPa,extraction temperature of 30℃and CO2 flow rate of 24 kg/h.The optimal conditions of ethanol extraction were reflux extraction for 3 times with 10-fold of 75%ethanol,1.5 h each time.Conclusion The optimal extraction process can be simple,repeatable and accurate,providing reference for the preparation of traditional Chinese medicine patch.

关 键 词：丹艾痛经贴 超临界CO2提取法 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]