ISSR分子标记结合相关分析快速预测远志中tenuifoliside C含量高低  

Prediction of Tenuifoliside C in Polygala tenuifolia with ISSR Markers and Correlation Analysis

作　　者：王海花 王丹丹[2,3] 景晓琦 张福生 秦雪梅[2] 马存根[4] WANG Hai-hua;WANG Dan-dan;JING Xiao-qi;ZHANG Fu-sheng;QIN Xue-mei;MA Cun-gen(Shanxi Pharmaceutical Vocational College,Taiyuan 030031, China;Modern Research Center for Traditional Chinese Medicine, Shanxi University,Taiyuan 030006,China;Shijiazhuang No.4 Pharmaceutical. Co.,Ltd.,Shijiazhuang 050021,China;Shanxi University of Chinese Medicine,Taiyuan 030024, China)

机构地区：[1]山西药科职业学院,山西太原030031 [2]山西大学中医药现代研究中心,山西太原030006 [3]石家庄四药有限公司,河北石家庄050021 [4]山西中医药大学,山西太原030024

出　　处：《中药材》2018年第8期1827-1831,共5页Journal of Chinese Medicinal Materials

基　　金：山西省重点研发计划重点项目(201603D3111003);山西省应用基础研究项目(201601D011064);国家中药标准化项目(ZYBZH-Y-JIN-34);中央本级重大增减支项目(2060302);国家科技支撑计划(2011BAI07B05)

摘　　要：目的:建立一种快速预测远志药材中化合物tenuifoliside C含量高低的方法,用于远志药材镇静安神药效强弱的初步评价。方法:采用UPLC法测定远志药材中化合物tenuifoliside C的含量,并对远志药材进行ISSR分析,通过相关分析筛选出与化合物tenuifoliside C含量高低相关的ISSR特异性条带,针对该ISSR条带设计特异性PCR引物。结果:筛选出4条与化合物tenuifoliside C含量相关的ISSR特异性条带,对其中1条特异条带(UBC835-1)进行胶回收、克隆测序分析,设计出1对特异性PCR引物(YZ-TC),可用于快速鉴别tenuifoliside C含量较低的远志药材样品。结论:采用ISSR分子标记结合相关分析技术,所设计出的特异性PCR引物可用于远志药材样品中tenuifoliside C含量高低的快速预测。Objective:To establish a method for rapidly predicting the content of tenuifoliside C in Polygala tenuifolia in order to preliminaryly evaluate the sedative effects of Polygala tenuifolia.Methods:UPLC method was used to determine the content of tenuifoliside C in Polygala tenuifolia.ISSR analysis was carried out in Polygala tenuifolia.ISSR-specific bands related to the content of tenuifoliside C were screened out by correlation analysis,and the specific PCR primers were designed for this ISSR band.Results:Four ISSR specific bands related to the content of tenuifoliside C were screened out and one of the specific bands(UBC835-1)was gel-recovered and cloned and then sequenced.One pair of specific PCR primers(YZ-TC)was designed,which could be used to identify Polygala tenuifolia samples with lower tenuifoliside C content quickly.Conclusion:The PCR primers designed by ISSR molecular marker combined with correlation analysis technology can be used to predict the content of tenuifoliside C in Polygala tenuifolia rapidly.

关 键 词：远志 ISSR 相关分析 Tenuifoliside C 特异性条带 

分 类 号：R284.1[医药卫生—中药学]