UPLC法测定翠云草中6种双黄酮类成分含量  

Quantitative Analysis of Six Biflavonoids in Selaginella uncinata by UPLC

作　　者：陈莹 肖凌[1] 龙小艳 黄志军 聂晶[1] CHEN Ying;XIAO Ling;LONG Xiao-yan;HUANG Zhi-jun;NIE Jing(Hubei Institute for Drug Control, Wuhan 430075 , China;Wuhan Lniversity of Technology, Wuhan 430070, China;Wuhan Jianmin Pharmaceutical Groups Co.,Ltd., Wuhan 430052,China)

机构地区：[1]湖北省药品监督检验研究院,湖北武汉430075 [2]武汉理工大学,湖北武汉430070 [3]武汉健民药业集团股份有限公司,湖北武汉430052

出　　处：《中药材》2018年第8期1910-1913,共4页Journal of Chinese Medicinal Materials

基　　金：国家食药监总局药化注册司项目;2016年国家中医药管理局中药标准行动计划(ZYBZH-C-HUB-20)

摘　　要：目的:建立UPLC法同时测定翠云草中2,3,2″,3″-四氢穗花杉双黄酮、2,3-二氢穗花杉双黄酮、穗花杉双黄酮、2,3-二氢-4′-甲氧基穗花杉双黄酮、白果黄素、罗波斯塔双黄酮-4′-甲醚等6种双黄酮类成分的含量。方法:采用ACQUITY UPLC BEH Shield RP_(18)色谱柱(100 mm×2.1 mm,1.7μm);以乙腈-0.1%甲酸溶液为流动相,梯度洗脱;流速:0.2 mL/min;柱温:30℃;检测波长:270 nm。结果:6种成分在各自范围内线性关系良好(r≥0.9997),平均加样回收率为98.24%～100.67%,RSD均小于2.0%。聚类分析结果显示,15批翠云草药材按产地分为湖北宜昌、广西南宁、湖南吉首三大类。结论:建立的含量测定方法灵敏、准确,可对翠云草药材质量进行控制。Objective:To establish an UPLC method for the content determination of six biflavonoids(2,3,2″,3″-tetrahydroamentoflavone,2,3-dihydroamentoflavone,amentoflavone,2,3-dihydro-4′-O-methyl-amentoflavone,bilobetin,and 4′-O-methyl-robustaflavone)in Selaginella uncinata.Methods:With ACQUITY UPLC BEH Shield RP18 column(100 mm×2.1 mm,1.7 μm),the mobile phase was acetonitrile-0.1% formic acid solution with gradient elution,the flow rate was 0.2 mL/min,the column temperature was 30 ℃,and the detection wavelength was 270 nm.Results:Six constituents showed good linear relationships with their own ranges(r≥0.9997),whose average sample recoveries were 98.24%~100.67% with the RSD was less than 2.0%.Cluster analysis results showed that Fifteen batches samples of Selaginella uncinata can be divided into Hubei Yichang,Guangxi Nanning,Hunan Jishou three regions.Conclusion:The established method is sensitive,accurate,and can be used to control the quality of Selaginella uncinata.

关 键 词：翠云草 UPLC 含量测定 统计分析 双黄酮 

分 类 号：R284.3[医药卫生—中药学]