多指标综合评分结合正交试验法优选瑶药牛白藤的提取工艺  

Extraction Technology Optimization of Yao Medicine Hedyotis hedyotidea by Orthogonal Test and Comprehensive Scoring Method

作　　者：黄健军[1] 岑芳 邓刚[1] HUANG Jian-jun;CEN Fang;DENG Gang(Guangxi University of Traditional Chinese Medicine, Nanning 530000, China;Chinese Medicine Hospital of Nanning, Nanning 530000, China)

机构地区：[1]广西中医药大学,广西南宁530000 [2]南宁市中医医院,广西南宁530000

出　　处：《中药材》2018年第8期1937-1940,共4页Journal of Chinese Medicinal Materials

基　　金：广西中医基础研究重点实验室(12-140-32-07);广西壮族自治区科学技术厅项目(桂知专151841-1);广西高校壮医方药基础与应用研究重点实验室(桂教科研[2016]6号zyfy201607)

摘　　要：目的:优选瑶药牛白藤的提取工艺。方法:以齐墩果酸含量、熊果酸含量和干膏率的综合评分为评价指标,对牛白藤三种提取方法(煎煮法、浸渍法、回流提取法)进行筛选;采用单因素试验和正交试验法,以乙醇浓度、提取时间和溶剂倍量为考察因素,优选牛白藤的提取工艺。结果:提取方法以回流提取法最优,最佳提取工艺为加12倍量75%乙醇回流提取120 min。结论:该工艺稳定可靠、重复性好,提取率高,可为牛白藤的后续研究提供参考。Objective:To optimize the extraction process of Yao Medicine Hedyotis hedyotidea.Methods:Based on the comprehensive score of the contents of oleanolic acid and ursolic acid and dry paste rate,the extraction technology(boiling method,soak method,reflux extraction method) of Hedyotis hedyotidea was optimized;the single factor test and orthogonal experimental design were employed to screen the extraction method by taking ethanol concentration,extraction time and solvent times as the evaluation factors.Results:The reflux extraction method was the best method;the optimum process conditions were 12 times liquid of 75% ethanol and extraction time was 120 min.Conclusions:The process is stable,repeatable and high extraction rate,which can provide reference for follow-up research of Hedyotis hedyotidea.

关 键 词：牛白藤 提取工艺 齐墩果酸 熊果酸 正交试验 

分 类 号：R283.6[医药卫生—中药学]