轮叶马先蒿的环烯醚萜苷成分及其抗补体活性  

Iridoid Glycosides Constituents of Pedicularis verticillata and Their Anti-complement Acticities

作　　者：王恒 邵明会 元思文 卢燕[2] 陈道峰[2] 王琪 WANG Heng;SHAO Ming-hui;YUAN Si-wen;LU Yan;CHEN Dao-feng;WANG Qi(Key Laboratory of Xinjiang Phytomedicine Resource and Utilization ,Ministry of Education,School of Pharmacy,Shihezi University,Shihezi 832002,China;School of Pharmacy,Fudan University,Shanghai 201203 ,China)

机构地区：[1]石河子大学药学院/新疆植物药资源利用教育部重点实验室,新疆石河子832002 [2]复旦大学药学院,上海201203

出　　处：《中药材》2018年第10期2349-2353,共5页Journal of Chinese Medicinal Materials

基　　金：新疆生产建设兵团科技援疆项目(2011AB033)

摘　　要：目的:研究轮叶马先蒿的环烯醚萜苷成分及其抗补体活性。方法:通过溶血实验,以抗补体活性为导向分离手段,对轮叶马先蒿各部位进行抗补体活性测试,采用各种色谱法进行分离,根据理化性质和波谱数据鉴定化合物结构,确定化合物的抗补体活性成分。结果:轮叶马先蒿正丁醇部位抗补体活性较强,从中分离鉴定了12个环烯醚萜苷类化合物,分别为:玉叶金花苷酸甲酯(1)、小米草苷(2)、龙船花苷(3)、桃叶珊瑚苷(4)、玉叶金花苷酸(5)、山栀苷甲酯(6)、京尼平苷(7)、7-去氧栀子新苷(8)、乙基胡桃苷(9)、乙基表桃叶珊瑚苷(10)、7-去氧-8-表番木鳖酸(11)、kankanoside A(12)。化合物5、6、11对经典途径的补体激活具有抑制作用。结论:其中,化合物7、8、12为首次从马先蒿属植物中分离得到。化合物1、3、6、9、10、11为首次从该植物中分离得到。化合物5显示出较强的抗补体活性,50%抑制溶血浓度(CH_(50))为164μg/mL,可作为今后开发天然补体抑制剂的候选化合物。Objective:To study the iridoid glycoside constituents of Pedicularis verticillata and their anti-complement activities.Methods:The anti-complement activities were tested in different parts of Pedicularis verticillata with the method of anti-complement activity oriented separation by hemolytic test.The compounds were isolated and purified by repeated silica gel,reversed-phase column chromatography and Sephadex LH-20.The structures of these compounds were elucidated by physico-chemical properties and spectral datum.The anti-complementary activity compounds were determined.Results:The n-butanol extraction of Pedicularis verticillata showed significant anti-complementary activity.Twelve compounds were isolated and identified as mussaenoside(1),euphroside(2),ixorioside(3),aucubin(4),mussaenosidic acid(5),shanzhiside methyl ester(6),geniposide(7),7-deoxygardoside(8),6-O-ethyl-aucubin(9),6-O-ethyl-epiaucubin(10),7-deoxy-8-epiloganic acid(11),kankanoside A(12).Compounds 5,6,11 inhibited the complement system towards the classical pathway.Conclusion:Compounds 7,8,12 are isolated from Pedicularis genus for the first time.Compounds 1,3,6,9,10,11 are isolated from Pedicularis verticillata for the first time.Compound 5 has stronger anti-complementary activity with the CH50 of 164 μg/mL,and can be used as a candidate compound for the development of natural complement inhibitors in the future.

关 键 词：轮叶马先蒿 环烯醚萜苷 抗补体活性 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]