耳叶牛皮消水溶性部位中一个新的生物碱苷  

One New Glycoalkaloid from the Water-soluble Fraction of Cynanchum auriculatum

作　　者：彭潇 吴莹莹 张宝[1,2] 马雪 孙佳[3] 王广成[3] 李勇军 PENG Xiao;WU Ying-ying;ZHANG Bao;MA Xue;SUN Jia;WANG Guang-cheng;LI Yong-jun(Guizhou Medical Universily ,Engineering Research Center for the Development and Application of Ethnic Medicine and Tradilioiuil Chinese Medicine( Ministry of Education)/State Key Laboratory of Functions and Applications of Medicinal Plants ,Guiyang 550004,China;Guizhou Medical Universily ,College of Pharmacy,Guiyang 550004,China;Guizhou Medical Universily ,Guizhou Provincial Key Laboratory of Pharmaceutics,Guiyang 550004,China)

机构地区：[1]贵州医科大学民族药与中药开发应用教育部工程研究中心/省部共建药用植物功效与利用国家重点实验室,贵州贵阳550004 [2]贵州医科大学药学院,贵州贵阳550004 [3]贵州医科大学贵州省药物制剂重点实验室,贵州贵阳550004

出　　处：《中药材》2018年第12期2820-2822,共3页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81760700);贵州省优青项目(201511);贵州省创新人才团队项目(20165613;20165677)

摘　　要：目的:对耳叶牛皮消水溶性部位的化学成分进行研究。方法:采用D-101大孔树脂、Sephadex LH-20、硅胶柱层析等色谱方法进行分离和纯化,并运用NMR、HR-ESI-MS和理化性质鉴定化合物的结构。结果:从耳叶牛皮消的水溶性部位分离得到2个化合物,分别鉴定为:6-[(β-D-吡喃木糖基)甲基]-3-羟基吡啶(1)、肉珊瑚苷元(2)。结论:其中,化合物1为新化合物,化合物2为首次从该植物中分离得到;化合物1对人慢性髓系白血病细胞(K562)及人前列腺癌细胞(PC3)表现出一定的抑制增殖活性。Objective:To study the chemical constituents from water-soluble fraction of Cynanchum auriculatum.Methods:The compounds were isolated and purified by D-101 macroporous resin,Sephadex LH-20,silica gel,and other methods.Their structures were elucidated by NMR,HR-ESI-MS and physicochemical properties.Results:Two compounds were isolated from water-soluble faction of Cynanchum auriculatum,and identified as 6-[(β-D-xylopyranosyl)methyl]-3-pyridinol(1),sarcostin(2).Conclusion:Compound 1 is a new compound,and has moderate inhibitory effect to human chronic myeloid leukemia cells(K562)and human prostate cancer cells(PC3),compound 2 is obtained from this plant for the first time.

关 键 词：耳叶牛皮消 化学成分 6-[(β-D-吡喃木糖基)甲基]-3-羟基吡啶 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]