蓝莓花青素诱导口腔癌KB细胞凋亡的实验研究  

Blueberry anthocyanin induces apoptosis in oral cancer KB cells: an laboratory study

作　　者：杨霞[1] 陈琦[1] 王利[1] Yang Xia;Chen Qi;Wang Li(Inner Mongolia Medical University, Inner Mongolia 010059, China)

机构地区：[1]内蒙古医科大学,内蒙古010059

出　　处：《北京中医药大学学报》2019年第4期312-317,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.81460496)~~

摘　　要：目的研究蓝莓花青素对人口腔癌KB细胞体内抑制作用,并从凋亡角度来探讨其机制。方法 20只BALB/c Nude免疫缺陷小鼠(裸鼠),构建人口腔癌KB细胞裸鼠异位移植瘤模型后,随机分为模型组,阳性对照组(顺铂组),蓝莓花青素低、中、高剂量组,每组4只。蓝莓花青素低、中、高剂量组分别给予蓝莓花青素25、50、100 mg/kg腹腔注射,模型组给予生理盐水10 mg/kg腹腔注射,顺铂组给予顺铂4 mg/kg腹腔注射。每3 d给药1次,连续给药21 d,末次给药后3 d处死动物。给药期间测取肿瘤长/短径,计算瘤体体积、抑瘤率;动物处死后摘除肿瘤组织,TUNEL法观察蓝莓花青素对移植瘤组织中细胞凋亡的影响;Western Blot检测瘤组织凋亡相关蛋白Bcl-2、Bax、C-caspase3和C-caspase9的表达水平。结果蓝莓花青素组裸鼠异位移植瘤体积较模型组均明显缩小,差异有统计学意义(P<0.05)。蓝莓花青素低、中、高剂量组抑瘤率分别为47.2%、46.2%、50.9%。蓝莓花青素处理的裸鼠异位移植瘤细胞凋亡率与模型组相比,差异具有统计学意义(P<0.01)。蓝莓花青素能明显上调凋亡相关蛋白C-caspase3、C-caspase9和Bax的表达(P<0.05),且呈一定的剂量依赖性,下调Bcl-2蛋白表达水平(P<0.05),并呈剂量依赖性下降。结论蓝莓花青素具有较好的抗肿瘤作用,可能通过调节内源性凋亡而抑制肿瘤细胞在体内增殖。Objective To study the inhibitory effect of blueberry anthocyanin on KB cells in human oral cancer and explore its mechanism from the perspective of apoptosis. Methods BALB/c Nude mice(n =20)with immuno deficiency were randomly divided into model, cisplatin group, and low-dose, mid-dose, and high-dose blueberry anthocyanin group(each n =4) after models of heterotopic xenograft of human oral cancer KB cells in nude mice were established. The blueberry anthocyanin group were given intraperitoneal injection blueberry anthocyanin at the doses of 25 mg/kg, 50 mg/kg and 100 mg/kg respectively;the model group were given saline in dose of 10 mg/kg;the cisplatin group were given cisplatin(4 mg/kg), once every three days for 21 days. All rats were executed three days after the last dose. After intraperitoneal injection of blueberry anthocyanin, the long/short diameters of the tumors were measured, and the tumor volume and tumor inhibition rate were calculated. The TUNEL method was used to observe the apoptosis of transplanted tumors in all groups after anthocyanin treatment. The expression of Bcl-2, Bax, C-caspase3 and C-caspase9 proteins was measured by using Western Blot Assay. Results Compared with the model group, the volume of heterotopic transplanted tumors in the blueberry anthocyanin group was significantly reduced, and the difference was statistically significant(P<0.05). The inhibition rates of low, medium and high dose blueberry anthocyanin were 47.2%, 46.2% and 50.9%, respectively. Compared with the model group, the apoptotic rate of heterotopic xenograft tumor cells treated with anthocyanin in blueberry had statistical significance(P<0.01). Blueberry anthocyanin could upregulate the expression of apoptosis-related proteins C-caspase3, C-caspase9 and Bax in a dose-dependent manner(P<0.05), and down-regulated the expression of Bcl-2 protein in a dose-dependent manner(P<0.05). Conclusion Blueberry anthocyanin has good anti-tumor effect, which may inhibit the proliferation of tumor cells in vivo by regulating endo

关 键 词：蓝莓花青素 口腔癌 凋亡 KB细胞 免疫缺陷小鼠 

分 类 号：R285.5[医药卫生—中药学]