不同时间针刺足三里穴对炎性大鼠脊髓、背根神经节中TRPV4蛋白表达的影响  

Effects of acupuncture at different time on TRPV4 protein expression in spinal cord and dorsal root ganglion of inflammatory rats

作　　者：周敏杰 刘楠楠 赵征宇[1] 蔡定均[1] Zhou Minjie;Liu Nannan;Zhao Zhengyu;Cai Dingjun(Chengdu University of Traditional Chinese Medicine Sichuan 610037, China)

机构地区：[1]成都中医药大学针灸推拿学院,四川610037

出　　处：《北京中医药大学学报》2019年第4期332-337,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.81774434;No.81373559)~~

摘　　要：目的研究不同时间针刺足三里穴对炎性大鼠脊髓及背根神经节中香草酸瞬时电位受体4(TRPV4)蛋白表达的影响。方法将54只清洁级的雄性SD大鼠驯化7 d后,进行基础痛阈筛选,然后按痛阈结果随机分组为造模组36只、空白组18只,造模组在右足足垫部皮内注射完全弗氏佐剂0.1 mL,空白对照组注射0.1 mL生理盐水。造模成功的大鼠又按照基础痛阈随机分为模型组18只和针刺组18只,每组按照不同的处理时间又分为ZT8(15:00)、ZT16(23:00)2个时间亚组,每组9只。在2个时间点进行处理,针刺组在相应时间点手针针刺患侧足三里30 min,其间每5 min捻转1 min,频率为120次/min,模型组、空白组捆绑固定30 min,不做其他处理,1次/d,共治疗7 d。在第7天治理结束后检测相应时间点大鼠的痛阈,痛阈检测后取材,提取大鼠腰椎4～6的脊髓及背根神经节,采用免疫组化的方法检测大鼠背根神经节以及脊髓中TRPV4的表达。结果①在ZT8时间点:模型组大鼠与空白组相比,背根神经节中TRPV4表达量显著增加(P<0.01);脊髓组织TRPV4蛋白表达量明显上调(P<0.05)。针刺组大鼠与模型组相比,针刺组背根神经节TRPV4表达量显著下降(P<0.01),脊髓组织TRPV4蛋白表达量无明显差异(P>0.05)。②在ZT16时间点:与空白组相比,模型组大鼠背根神经节TRPV4表达量明显增加(P<0.05),脊髓组织TRPV4蛋白表达量明显上调(P<0.01)。与模型组相比,针刺组大鼠背根神经节TRPV4表达量无明显差异(P>0.05),脊髓组织TRPV4蛋白表达量降低(P<0.05)。结论炎性痛能够诱发大鼠脊髓和背根局部神经节TRPV4蛋白表达的上调;针刺足三里穴能够调节炎性痛大鼠脊髓与背根神经节TRPV4的蛋白含量。Objective To study the effects of acupuncture at ST36(Zusanli) on the protein expression of transient vanillin receptor potential channel 4(TRPV4) in the spinal cord and dorsal root ganglia of inflammatory rat. Methods A total of 54 male SD rats of clean grade were acclimated for 7 days, and the basic pain thresholds were screened. Then, these rats were randomly divided into model(n =36) and control(n =18) groups based on the pain threshold results. 0.1 mL of complete Freund’s adjuvant was injected internally, and 0.1 mL of physiological saline was injected into the blank control group. Rats with successful modeling were further divided into model group(n =18) and model+acupuncture group(n =18). Each group was needled at ZT8(15:00) or ZT16(23:00) as two different treatment time subgroup(n =9). At two time points, the needle group was needled at the corresponding time point for 30 minutes of the affected side, and the frequency was 120 times/min every 5 minutes. The model group and the control group were fixed for 30 minutes without any further treatment. After the end of the treatment on the 7 th day, the pain threshold of the rats at the corresponding time points was detected. The spinal cord and dorsal root ganglia of the lumbar vertebrae 4-6 were extracted, and the rat dorsal root ganglion was detected by immunohistochemistry. Section and expression of TRPV4 in the spinal cord. Results ①At the time of ZT8: compared with the blank group, the expression level of TRPV4 in the dorsal root ganglion increased significantly in the model group(P<0.01);the expression level of TRPV4 protein in spinal cord tissue was significantly up-regulated(P<0.05). Compared with the model group, the TRPV4 expression level in the dorsal root ganglion of the model group was significantly decreased(P<0.01). There was no significant difference in the TRPV4 protein expression level in the spinal cord tissue(P>0.05).② At the time of ZT16: compared with the blank group, the expression level of TRPV4 in the dorsal root ganglion of the

关 键 词：针刺 时间特征 炎性疼痛 香草酸瞬时电位受体4 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]