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作 者:于非 王禹 Yu Fei;Wang Yu(Horticultural Sub-academy of Heilongjiang Academy of Agriculture Sciences,Harbin,150069)
机构地区:[1]黑龙江省农科院园艺分院
出 处:《中国林副特产》2019年第3期10-13,共4页Forest By-product and Speciality in China
基 金:哈尔滨市科学技术局项目(2017RAQYJ117)
摘 要:根据目前丽格海棠组织培养快繁现状,研究了以MS培养基为基础附加不同种类和配比植物激素对丽格海棠组织培养中不定芽再生、不定芽继代增殖和生根的影响。结果表明:丽格海棠不定芽再生的最佳植物激素配比为MS+1mg/L6-BA+0.1mg/LTDZ或MS+1mg/L6-BA+0.1mg/L2.4-D;不定芽继代增殖的最佳植物激素配比为MS+2mg/L6-BA+0~0.1mg/LNAA;组培苗生根的最佳植物激素配比为MS+0.1~0.3mg/LIBA。According to the present situation of tissue culture and rapid propagation of Begonia regia,the effects of different kinds and proportions of plant hormones on adventitious bud regeneration,adventitious bud subculture and rooting in tissue culture of Begonia Rieger were studied on the basis of MS medium. The results show that:The optimum phytohormone ratio for adventitious bud regeneration of Begonia Rieger was MS+1mg/L6-BA+0.1mg/LTDZ or MS+1mg/L6-BA+0.1mg/L2.4-D. The optimum ratio of plant hormones for adventitious bud subculture was MS+2 mg/L 6-BA+0-0.1 mg/L NAA. The optimum ratio of plant hormones for rooting of tissue culture seedlings was MS + 0.1-0.3 mg/L IBA.
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