MMP-9和TIMP-1基因多态性与糖尿病足的相关性研究  

Association of the MMP-9 and TIMP-1 gene polymorphism with diabetic foot

作　　者：陈占玲[1] 孙庆凯[1] 陈松锦[1] 张秀薇[1] CHEN Zhanling;SUN Qingkai;CHEN Songjin;ZHANG Xiuwei(Department of Endocrinology,Dongguan People′s Hospital,Dongguan,Guangdong 523059,China)

机构地区：[1]东莞市人民医院内分泌科

出　　处：《现代医药卫生》2019年第11期1627-1629,共3页Journal of Modern Medicine & Health

基　　金：广东省东莞市科技局社会科技发展(一般)项目(201750715001304)

摘　　要：目的研究基质金属蛋白酶-9(MMP-9)和组织金属蛋白酶抑制物-1(TIMP-1)基因多态性与糖尿病足的相关性。方法采集健康者(72例)、2型糖尿病无糖尿病足患者(69例)和2型糖尿病糖尿病足患者(65例)外周抗凝血3mL,提取外周血基因组DNA,采用高分辨率熔解曲线技术检测MMP-9基因-1562C>T和TIMP-1基因-9830T>G位点基因型和等位基因频率。结果糖尿病足患者MMP-9基因-1562位点T等位基因频率明显高于健康者和无糖尿病足患者(P<0.05),TIMP-1基因-9830T>G基因型和基因频率与健康者、无糖尿病足患者比较差异无统计学意义(P>0.05)。结论 MMP-9基因-1562T等位基因型可能是糖尿病足的易感因素。Objective To investigate the association of matrix metalloproteinase-9(MMP-9) and tissue inhibitors of matrix metalloproteinases-1(TIMP-1) gene polymorphism with diabetic foot. Methods Peripheral blood genomic DNA was extracted from healthy subjects(72 cases), patients with type 2 diabetes but without diabetic foot(69 cases) and patients with type 2 diabetes and diabetic foot(65 cases).Genotype and allelic frequencies of MMP-9 gene -1562C>T and TIMP-1 gene -9830T>G loci were detected by using high resolution melting curve technique. Results The T allele frequency of MMP-9 gene -1562 in patients with diabetic foot was significantly higher than healthy subjects and patients without without diabetic foot( P <0.05).There was no significant difference in TIMP-1 gene -9830 T>G genotype and gene frequency among the three groups( P >0.05). Conclusion MMP-9 gene-1562 T allele might be a susceptible factor for diabetic foot.

关 键 词：糖尿病足 基质金属蛋白酶-9 组织金属蛋白酶抑制剂-1 高分辨率熔解曲线 基因多态性 

分 类 号：R587.2[医药卫生—内分泌]