静脉注射ANP对阿尔泰大尾羊血脂代谢相关生化指标的影响  

Effects of Intravenous Infusion of ANP on Biochemical Indexes Related to Blood Lipid Metabolism in Altay Big-Tailed Sheep

作　　者：杨光[1] 韩冰[2] 李翔宇 马菁菁 王蕾 陈勇[1] YANG Guang;HAN Bing;LI Xiang-yu;MA Jing-jing;WANG Lei;CHEN Yong(College of Animal Science,Xinjiang Agriculture University,Urumqi 830052,China;Key Laboratory of Animal Biotechnology of Xinjiang,Biotechnological Research Center,Xinjiang Academy of Animal Sciences,Urumqi 830011,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052 [2]新疆畜牧科学院生物技术研究中心,新疆维吾尔自治区动物生物技术重点开放实验室,乌鲁木齐830011

出　　处：《新疆农业大学学报》2018年第6期429-433,共5页Journal of Xinjiang Agricultural University

基　　金：国家自然科学基金项目(U1603107)

摘　　要：为研究心房利钠肽(ANP)对阿勒泰大尾羊体脂代谢的短期效应,本试验选取8只1.5岁雌性阿勒泰大尾羊静脉滴注心房利钠肽,采用自身对照试验设计,对照期静脉滴注生理盐水,试验期按照1.125μg/kg体重的剂量静脉滴注合成绵羊ANP。连续滴注4d,在第4天分别于滴注前(即0min)和滴注开始后15、30、45、60、90和120min采集血液分离血浆用于测定脂代谢相关生化指标。结果表明,静脉滴注ANP对阿勒泰大尾羊血浆中低密度脂蛋白胆固醇(LDL-C)与高密度脂蛋白胆固醇(HDL-C)无显著影响(P>0.05),甘油三酯(TG)、游离脂肪酸(FFA)、甘油含量均有不同程度的增加。在滴注ANP15min时,TG含量较对照期增加了21.11%(P<0.01),之后TG含量随滴注时间呈下降趋势。滴注ANP15min后,试验期血浆中FFA水平均高于对照期,但仅在120min时显著高于照期,血浆FFA水平提高了11.36%(P<0.05)。在滴注45min时,试验期血浆中甘油含量相对于对照期含量增加了36.06%(P<0.05),随后恢复正常水平。由此可见,ANP在一定程度上增加了阿勒泰大尾羊的脂肪分解。In order to study the short-term effect of atrial natriuretic peptide (ANP) on body fat metabolism in Altay big-tailed sheep,eight 1.5-year-old female sheep were intravenously dripped with atrial natriuretic peptide (ANP).The self-control design was used,in the control period,saline was intravenously dripped,and in the experimental period,synthesized sheep ANP was intravenously dripped at a dose of 1.125 μg/kg body weight.Saline or ANP was continuously dripped for 4 days.On the fourth day,blood plasma was collected before drip (0 min) and 15,30,45,60,90 and 120 minutes after drip to determine lipid metabolism-related biochemical indexes.The results showed that there was no significant effect on plasma low density lipoprotein cholesterol(LDL-C)and high density lipoprotein cholesterol(HDL-C)(P >0.05) by intravenous drip of ANP.The contents of triglyceride (TG),free fatty acid (FFA) and glycerol increased to varying degrees.The content of TG increased by 21.11%(P <0.01) when ANP was dripped for 15 minutes,and then decreased with the dripping time.After 15 minutes of ANP infusion,plasma FFA level was higher than that of the control period,but it was significantly higher only at 120 minutes,and the FFA level increased by 11.36%(P < 0.05).The glycerol content increased by 36.06%(P <0.05) during the experimental period compared with that of the control period at 45 min,and then returned to normal level. It can be seen that ANP infusion increased lipolysis of Altay big-tailed sheep to a certain extent.

关 键 词：心房利钠肽 阿勒泰大尾羊 血脂 脂解 

分 类 号：S826[农业科学—畜牧学]