红景天乙醇提取物调节Lewis肺癌荷瘤小鼠肿瘤浸润T细胞数量并增强抗肿瘤免疫效应  

Ethanol extract of Rhodiola rosea L. regulates the number of tumor infiltrating T cells to enhance antitumor effect in Lewis lung cancer-bearing mice

作　　者：张燕丽 张雪伟 岳秋娟 文泽馨 张敏 ZHANG Yanli;ZHANG Xuewei;YUE Qiujuan;WEN Zexin;ZHANG Min(Key Laboratory of Life Sciences, Colleges of Medicine, Xizang Minzu University, Xianyang 712082;Department of Clinical Medicine, Fujian Medical University, Fuzhou 350004, China)

机构地区：[1]西藏民族大学医学部生命科学高校重点实验室,陕西咸阳712082 [2]福建医科大学临床医学部,福建福州350004

出　　处：《细胞与分子免疫学杂志》2019年第2期103-108,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81560668;81860719);西藏自治区科技厅项目(XZ2017ZRG-59;XZ2017ZRG-72);西藏民族大学重大培育项目(15myZP02);西藏民族大学校内科研项目(17MDQ04);西藏民族大学教学改革与研究项目(2017359);西藏高校青年教师创新支持计划项目(QCZ2016-36)

摘　　要：目的探讨藏药红景天(RRL)的抗肿瘤免疫功能。方法将Lewis肺癌荷瘤小鼠随机分为生理盐水组、 500 mg/kg RRL乙醇提取物处理组和10 mg/kg环磷酰胺(CTX)处理组,处理10 d。计算小鼠生存率和肿瘤生长抑制率;流式细胞术检测肿瘤浸润的CD4^+T、 CD8^+ T细胞数量及FOXP3^+调节性T细胞(Treg)占CD4^+CD25^+Treg的比例; ELISA检测荷瘤小鼠血清中白细胞介素2 (IL-2)和γ干扰素(IFN-γ)水平,乳酸脱氢酶(LDH)释放法检测脾细胞毒性T淋巴细胞(CTL)活性。结果 RRL乙醇提取物处理的Lewis荷瘤小鼠生存率显著提高,肿瘤生长受到抑制,肿瘤浸润的CD4^+T细胞和CD8^+T细胞数量增加, FOXP3^+ Treg占CD4^+CD25^+Treg的比例降低。荷瘤小鼠血清IFN-γ和IL-2水平提高,脾脏CTL的杀伤能力增强。结论 RRL乙醇提取物通过调节免疫细胞数量和功能增强抗肿瘤免疫效果。Objective To explore the anti-tumor immune function of the Tibetan medicine Rhodiola rosea L.(RRL). Methods Lewis lung cancer-bearing mice were randomly divided into normal saline group, 500 mg/kg RRL ethanol extract treatment group, and 10 mg/kg cyclophosphamide(CTX) treatment group. All the groups underwent the treatment for 10 days. The mouse survival rate and tumor inhibitory rate were calculated. Additionally, the numbers of CD4^+T and CD8^+ T cells of tumor infiltrating lymphocytes as well as the proportion of FOXP3^+ regulatory T cells(Tregs) in the CD4^+CD25^+Tregs were detected by flow cytometry. Besides, the serum levels of interleukin 2(IL-2) and γ-interferon(IFN-γ) in the tumor-bearing mice were examined through ELISA, and the spleen cytotoxic T lymphocytes(CTL) activity was detected by the lactic dehydrogenase(LDH) release assay. Results Lewis tumor-bearing mice treated with the ethanol extract of RRL showed remarkably enhanced survival rate and inhibited tumor growth. Furthermore, the number of tumor infiltrating CD4^+T and CD8^+ T cells increased, while the proportion of FOXP3^+ Tregs in the CD4^+CD25^+Tregs showed a declined tendency. Meanwhile, the serum IFN-γ and IL-2 levels in Lewis tumor-bearing mice increased, and the killing capacity of spleen CTL was enhanced. Conclusion The ethanol extract of RRL has a positive role in enhancing the anti-tumor immunity by regulating the number and function of immunocytes.

关 键 词：红景天(RRL) 调节性T细胞(Treg) FOXP3+ TREG 抗肿瘤效应 

分 类 号：R285.5[医药卫生—中药学] R392.11[医药卫生—中医学]