抗生素诱导的小鼠肠动力减弱与肠胶质细胞过度活化相关  

Gut mobility attenuation induced by antibiotics is associated with overactivation of enteric glial cells in mice

作　　者：谢韬[1] 加潇坤 陈木彬 XIE Tao;JIA Xiaokun;CHEN Mubin(Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China)

机构地区：[1]南方医科大学珠江医院神经外科,广东广州510280

出　　处：《细胞与分子免疫学杂志》2019年第3期199-205,共7页Chinese Journal of Cellular and Molecular Immunology

基　　金：广东省科技厅项目(2017A020215044)

摘　　要：目的探讨抗生素处理小鼠肠动力与肠胶质细胞活化的关系。方法通过联合抗生素处理获得低肠道菌群的小鼠,通过胭脂红溶液灌胃后记录色素排出时间以及相同时间内排出粪便颗粒,比较抗生素组与对照组小鼠的肠动力;采用免疫荧光技术比较两组小鼠肌间神经丛内胶质细胞与神经元的形态与分布,采用实时荧光定量PCR检测两组小鼠结肠与回肠组织内肠胶质细胞活化与功能相关的基因胶质纤维酸性蛋白(GFAP)、胶质细胞源性神经营养因子(GDNF)、 S100B、神经生长因子(NGF)的mRNA水平,采用流式细胞术检测小鼠肠黏膜固有层淋巴细胞内调节性T细胞(Treg)和分泌白细胞介素17(IL-17)的Th17细胞的比例。结果抗生素处理后的小鼠肠道菌群数量显著降低,盲肠体积膨大,结肠萎缩变短,肠动力减弱,结肠内胶质细胞GFAP、 GDNF、 S100B、 NGF的mRNA水平增加,而结肠黏膜固有层内Treg和Th17细胞比例均降低。结论抗生素处理后肠动力的降低可能与肠胶质细胞的过度活化相关,且抗生素处理后肠胶质细胞的活化与肠道炎症无关。Objective To explore the relationship between gut motility and enteric glial cell activation in antibiotics-treated mice. Methods We got the mice with lower intestinal flora through antibiotics(ABX) cocktails treatment, and compared gut mobility between ABX mice and control mice by detecting the time of carmine red going through gastrointestinal tract and the fecal pellets in the same time. Immunofluorescence histochemistry was used to detect the distribution of enteric glial cells and neurons in myenteric nerve plexus of the two groups of mice. Real-time fluorescence quantitative PCR was used to detect the mRNA levels of glial fibrillary acidic protein(GFAP), glial cell-derived neurotrophic factor(GDNF), S100B and nerve growth factor(NGF), which were related to enteric glial cell activation and function both in colon and ileum. The population of regulatory T cells(Tregs) and Th17 cells in intestinal lamina propria was assessed by flow cytometry. Results Antibiotics treatment induced a significant reduction of intestinal flora in the mice, which was accompanied by cecum volume expansion, colon shortening and gut motility attenuation. In addition, mRNA levels of GFAP, GDNF, S100B, and NGF in colon of ABX mouse gastrointestinal tract increased, while the population of Tregs and Th17 cells in colonic lamina propria decreased. ConclusionThe attenuation of gut motility induced by antibiotics treatment may be related to the excessive activation of enteric glial cells. However, the activation of enteric glial cells after antibiotics treatment is not related to intestinal inflammation.

关 键 词：肠动力 抗生素 肠胶质细胞 肠道菌群 

分 类 号：R978.1[医药卫生—药品] R574[医药卫生—药学]