大豆GmGW2基因的生物信息学分析  

Bioinformatics Analysis of Gene GmGW2 in Soybean

作　　者：吕金东[1] 刘灿 屈亦潇 陈汉谋 杨翊晖 丁晨 郭蓓 LYU Jin-dong;LIU Can;QU Yi-xiao;CHEN Han-mou;YANG Yi-hui;DING Chen;GUO Bei(College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China;College of Biological Science and Engineering,Beijing University of Agriculture,Beijing 102206,China;Key Laboratory of Urban Agriculture ( North China),Ministry of Agriculture and Rural Affairs of the People's Republic of China,Beijing 102206,China)

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]北京农学院生物科学与工程学院,北京102206 [3]中华人民共和国农业农村部华北都市农业重点实验室,北京102206

出　　处：《大豆科学》2019年第3期379-384,共6页Soybean Science

摘　　要：GW2基因在多种植物中发挥调控籽粒性状的功能,为预测分析大豆中GW2基因的功能,前期通过同源克隆的方法从大豆中成功克隆出GmGW2基因的cDNA序列。为了继续开展深入研究,本试验从大豆中黄10号中克隆出GmGW2基因的全长DNA序列并进行了较为详细的生物信息学分析。结果表明:GmGW2全长8 467 bp,包含8个外显子和7个内含子,编码431个氨基酸。预测是一个不稳定亲水蛋白,定位于细胞核或细胞质中,不含跨膜结构域及信号肽,具有Zinc finger和RING-type结构域。二级、三级结构预测分析显示,α-螺旋及不规则卷曲是其整体蛋白质结构中的主要组成结构元件。利用该基因的DNA序列,构建进化树分析得出该基因与木豆、相思子、鹰嘴豆亲缘关系较近。根据以上分析结果推测GmGW2基因可能也具有调控大豆籽粒发育的功能。此研究旨在为进一步挖掘和验证GmGW2基因功能奠定基础,为大豆产量育种工作提供有益信息。The GW2 gene in diverse plants can control grain width and grain weight. In order to predicet and analysis the function of GW2 gene in soybean, the cDNA sequence of the GmGW2 gene was successfully cloned from soybean by the method of homologous cloning. In order to continue the in-depth study, the full-length DNA sequence of GmGW2 gene was cloned from soybean Zhonghuang 10 and detailed bioinformatics analysis was carried out in this experiment. The results showed that the cloned GmGW2 was consisted of 8 exons and 7 introns, encoding 431 amino acids, which was predicted to be located in the nucleus or cytoplasm, without transmembrane domain and signal peptide, and it was an unstable hydrophilic protein with a Zinc finger, RING-type domain. The secondary and third structure prediction analysis showed that α-helix and irregular curl were the main structural elements in the overall protein structure. Phylogenetic tree showed that the gene was closely related to Cajanus cajan, Abrus precatorius and Cicer arietinum. GmGW2 gene may also play a regulational function in soybean seed traits above the results. The results of this study laid the foundation for further digging and verifying the function of GmGW2 gene, aiming to provide useful information for soybean yield breeding.

关 键 词：大豆 中黄10号 GmGW2 生物信息学分析 

分 类 号：S565.1[农业科学—作物学]