益气复脉合剂治疗异丙肾上腺素所致心律失常的实验研究  

Experimental study on Yiqifumai mixture in treatment of arrhythmia induced by isoproterenol

作　　者：李昊娲[1] 武乾[2] 石晓路[2] 林谦[3] Li Haowa;Wu Qian;Shi Xiaolu;Lin Qian(Department of Traditional Chinese Medicine,Beijing Anzhen Hospital,Capital Medical University,Beijing 100029,China;Medical Experimental Center of Chinese Academy of Traditional Chinese Medicine,Beijing 100700,China;Department of Cardiology,Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China)

机构地区：[1]首都医科大学附属北京安贞医院中医科,北京100029 [2]中国中医科学院医学实验中心,北京100700 [3]北京中医药大学东方医院心内科,北京100078

出　　处：《中国中西医结合急救杂志》2019年第2期139-142,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：北京市科技计划课题(Z141100002214005).

摘　　要：目的评价益气复脉合剂对异丙肾上腺素所致室性心律失常的影响。方法选择40只自发性高血压大鼠(SHR),按随机数字表法分为对照组和中药组,每组20例。对照组灌胃蒸懈水3.48 mL·kg^-1·d^-1,中药组灌胃益气复脉合剂(组成:党参、黄连、半夏、鬼箭羽、川茸、丹参、赤芍、白芍、炙甘草、酸枣仁、远志)3.48 mL-kg-1-^1,两组均连续给药7 d。于末次给药1 h后颈部皮下注射异丙肾上腺素100 mg/kg诱发心律失常。心电遥测2 h,记录两组单发室性期前收缩(单发室早,SP)、成对室早(PP)、室性心动过速(VT)的发生率、出现次数及出现时间。结果心电遥测lh、2h,对照组和中药组SP发生率(SPR)、PP发生率(PPR)、VT发生率(VTR)比较差异均无统计学意义〔1 h SPR 为 90%(18/20)比 80%(16/20 ),PPR 为 65%(13/20)比 65%( 13/20),VTR 为 45%(9/20)比 40%(8/20);2 h SPR 为 100%(20/20)比 100%(20/20),PPR 为 75%(15/20)比 75%(15/20),VTR为65%( 13/20)比60%( 12/20),均P>0.05〕。心电遥测1 h,中药组SP次数显著低于对照组〔次:10.00(4.00,11.00)比16.00(8.50,42.50),P<0.05〕;心电遥测2 h,中药组出现SP、PP及VT次数均显著低于对照组〔SP(次):27.00(15.50, 38.00)比 37.50(24.00,74.50), PP(次):5.00(3.00,8.00)比 7.00(5.00, 11.00), VT(次):2.50(1.25,4.00)比7.00(4.50, 11.00),均P<0.05〕。心电图遥测lh、2h后,中药组与对照组SP、PP、VT出现时间稍长于对照组,但差异均无统计学意义〔1 h:SP(min)为 4.35(3.65,9.90)比 3.66(1.12,9.52),PP(min)为 35.56(26.78,46.42)比 23.39(11.74,43.42), VT(min)为 22.31 (6.25,30.02)比 14.27(8.79,31.38);2 h:SP(min)为 7.06(3.65,12.29)比 4.09(1.38, 14.11), PP(min)为 46.40(33.88, 71.39)比 33.81 (14.54, 46.20), VT(min)为 75.49(59.37,96.63)比60.55(24.65, 86.48),均P>0.05 L结论中药益气复脉合剂具有拮抗异丙肾上腺素诱发心律失常的作用,且随时间延长作用更显著。Objective To evaluate the effect of Yiqifumai mixture on ventricular arrhythmia induced by isoproterenol. Methods Forty spontaneous hypertensive rats (SHR) were randomly divided into a control group and a Chinese medicine (TCM) group with 20 rats in each group. The control group was given distilled water 3.48 mL ? kg-1 ? d"1, and the TCM group was given Yiqifumai mixture (composition: Codonopsis pilosula, Rhizoma coptidis, Pineilia ternate, Euonymus alatus, Rhizome of chuanxiong, Salvia miltiorrhiza, Radix paeoniae rubra, Radix paeonia alba, Licorice, Zizyphus jujuba, Polygala tenuifolia) 3.48 mL· kg-1 · d_l, both groups were administered continuously for 7 days. Arrhythmia was induced by subcutaneous injection of isoproterenol 100 mg/kg into the neck 1 hour after the last administration in both groups. ECG telemetry was carried out for 2 hours to record whether single premature ventricular contraction (SP), paired premature ventricular contraction (PP) and ventricular tachycardia (VT) occurred in the control group and the TCM group, and the incidences, numbers and times of their occurrences were registered. Results There were no statistical significant differences in SP incidence (SPR), PP incidence (PPR), VT incidence (VTR) between the control group and TCM group at 1 hour and 2 hours [1 hour SPR was 90%(18/20) vs. 80%(16/20), PPR was 65%(13/20) vs. 65%(13/20), VTR was 45%(9/20) vs. 40%(8/20);2 hours SPR was 100%(20/20) vs. 100%(20/20), PPR was 75%(15/20) vs. 75%(15/20), VTR was 65%(13/20) vs. 60%(12/20);all P > 0.05]. After 1 hour of ECG telemetry, the number of SP in the TCM group was significantly lower than that in the control group [numbers: 10.00 (4.00, 11.00) vs. 16.00 (8.50, 42.50), P < 0.05];after 2 hours of ECG telemetry, the numbers of SP, PP and VT in the TCM group were significantly lower than those in the control group [SP (numbers), 27.CX)(15.50, 38.00) vs. 37.50 (24.00, 74.50), PP (numbers), 5.00 (3.00, 8.00) vs 7.00 (5.00, 11.00), VT (numbers), 2.50 (1.25, 4.00) vs. 7.00 (4.50, 11.00), all P

关 键 词：益气复脉合剂 室性期前收缩 心电遥测 异丙肾上腺素 

分 类 号：R285.5[医药卫生—中药学]