血必净对百草枯诱导人肾近曲小管上皮细胞-2氧化应激和炎症水平的影响  

Effect of Xuebijing on paraquat-induced cell oxidative stress and inflammation level in human renal proximal convoluted tubular epithelial

作　　者：张万里 余兴蓉 田昕[3] 洪广亮[2] 曹凯强 项公强 卢中秋[2] Zhang Wanli;She Xingrong;Tian Xin;Hong Guangliang;Cao Kaiqiang;Xiang Gongqiang;Lu Zhongqiu(Wenzhou Longwan First People's Hospital,Wenzhou 325024,Zhejiang,China;Department of Emergency,the FirstAffiliated Hospital of Wenzhou Medical Universityf Wenzhou 325000,Zhejiang,China;The Fifth Hospital Affiliated toWenzhou Medical University,Lishui 323000,Zhejiang,China)

机构地区：[1]温州市龙湾区第一人民医院,浙江温州325024 [2]温州医科大学附属第一医院急诊医学中心,浙江温州325000 [3]温州医科大学附属第五医院,浙江丽水323000

出　　处：《中国中西医结合急救杂志》2019年第2期143-146,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：浙江省温州市科技计划项目(Y20160310);浙江省中医药重点学科计划(2012-XK-A28);浙江省“十二五”重点学科建设项目(2012-207);浙江省医学创新学科建设项目(11-CX26).

摘　　要：目的观察血必净注射液对百草枯诱导的人肾近曲小管上皮细胞-2(HK-2)氧化应激和炎症水平的影响。方法常规培养HK-2细胞,按随机数字表法分为空白对照组、百草枯中毒模型组、血必净预处理组、血必净对照组。采用高效液相色谱法(HPLC)检测细胞内百草枯含量;采用化学比色法检测超氧化物歧化酶(SOD)和丙二醛(MDA)水平;采用酶联免疫吸附试验(ELISA)检测白细胞介素-6 (IL-6)和肿瘤坏死因子-a(TNF-a)水平。结果随时间延长,百草枯中毒模型组和血必净预处理组HK-2细胞内百草枯含量逐渐升高,作用48 h达峰值,且血必净预处理组HK-2细胞内百草枯含量较百草枯中毒模型组明显降低(mg/L :4.26±0.20比5.77±0.18, P<0.05)。与空白对照组比较,百草枯中毒模型组MDA.TNF-a和IL-6含量明显升高[MDA(nmo<mg): 4.47±0.10 比 2.21 ±0.08, TNF-a (ng/L): 206.91 ± 13.22 比 98.14±5.67), IL-6(ng/L):253.33±5.22 比 116.97± 13.54,均 P<0.05〕,SOD 活性明显降低(U/mg : 33.30±0.62 比 41.58±0.17, P<0.05)。与百草枯中毒模型组比较,血必净预处理组HK-2细胞内MDA、TNF- a和IL-6含量明显降低〔MDA(nmol/mg):2.92 ±0.17 比 4.47 ±0.10, TNF-a (ng/L): 166.29 ±15.47 比 206.91 ± 13.22, IL-6 (ng/L ): 209.39 ±3.18 比253.33 ±5.22,均 P<0.05〕,SOD 活性明显升高(U/mg : 38.10±0.67 比 33.30±0.62, P<0.05)。结论血必净可降低HK-2细胞内百草枯含量以及细胞氧化应激和炎症因子水平。Objective To investigate the effect of Xuebijing on paraquat-induced oxidative stress and inflammation level in human kidney cell line-2 (HK-2) cells. Methods The HK-2 cells were routinely cultured and divided into blank control group, paraquat poisoning model group, Xuebijing injection pretreatment group and Xuebijing control group according to random number table method. The concentration of paraquat in HK-2 cells were measured by high performance liquid chromatography (HPLC);the activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) were measured by chemical colorimetry method;the levels of interleukin-6 (IL-6) and tumor necrosis factor- a (TNF- a) were detected by enzyme linked immunosorbent assay (ELISA). Results With prolonged treatment, paraquat concentration gradually increased in HK-2 cells in the paraquat poisoning model group and the Xuebijing injection pretreatment group, reaching the peak value after treatment for 48 hours, moreover, the concentration of paraquat in HK-2 in the Xuebijing injection pretreatment group was significantly lower than that in the paraquat poisoning model group (mg/L: 4.26 ± 0.20 vs. 5.77 ±0.18, P < 0.05). Compared with the blank control group, the contents of MDA, TNF- a and IL-6 of paraquat poisoning model group were obviously elevated [MDA (nmol/mg): 4.47 ±0.10 vs. 2.21 ±0.0& TNF-a (ng/L): 206.91 ± 13.22 vs. 98.14±5.67, IL-6 (ng/L): 253.33±5.22 vs. 116.97± 13.54, all P < 0.05], and the activity of SOD was significantly reduced (U/mg: 33.30 + 0.62 vs. 41.58 ±0.17, P < 0.05). Compared with paraquat poisoning model group, the contents of MDA, TNF- a and IL-6 in Xuebijing injection pretreatment group were obviously decreased [MDA (nmol/mg): 2.92±0.17 vs. 4.47 ±0.10, TNF- a (ng/L): 166.29 ± 15.47 vs. 206.91 ± 13.22, IL-6 (ng/L): 209.39 ± 3vs. 253.33 ±5.22, all P < 0.05], and the activity of SOD was significantly increased (U/mg: 3&10 ±0.67 vs. 33.30 ±0.62, P < 0.05) in the paraquat pretreatment group. Conclusion Xuebijing could reduce th

关 键 词：血必净 百草枯 氧化应激 炎症 人肾近曲小管上皮细胞-2 

分 类 号：R692.502[医药卫生—泌尿科学]