在中国东北汉族人群中验证复合DIP-STR遗传标记  

Verification of Composite DIP-STR Genetic Markers in Northeast China’s Han Population

作　　者：金鑫 许思娴 夏明英 杨淑萍 殷才湧 李士林 JIN Xin;XU Sixian;XIA Mingying;YANG Shuping;YIN Caiyong;LI Shilin(MOE(Ministry of Education)’s Key Laboratory of Contemporary Anthropology,Department of Anthropology and Human Genetics,School of Life Sciences,Fudan University,Shanghai 200438,China;Library,Nanjing Tech University,Nanjing 210000,China;State Key Laboratory of Genetic Engineering Based at Fudan University,Shanghai 200438,China;Fudan-Taizhou Institute of Health Sciences,Taizhou 225300,Jiangsu,China;Shanghai Key Laboratory of On-site Criminal Evidence,Shanghai 200000,China)

机构地区：[1]复旦大学生命科学学院,现代人类学教育部重点实验室,人类学与人类遗传学系,上海200438 [2]南京工业大学图书馆,南京工业大学,南京210000 [3]遗传工程国家重点实验室,上海200438 [4]复旦大学泰州健康科学研究院,江苏泰州225300 [5]上海市犯罪现场证据重点实验室,上海200000

出　　处：《刑事技术》2019年第3期207-212,共6页Forensic Science and Technology

基　　金：“十二五”国家科技支撑计划项目(No.2012BAK16B01);国家自然科学基金(No.30901701、31071096、81172908);上海市犯罪现场证据重点实验室开放课题

摘　　要：目的建立适宜的复合扩增体系,并在中国东北汉族人群中验证所选择的DIP-STR遗传标记,以推动DIP-STR遗传标记应用于实际办案。方法参照DianaHall等人设计的单遗传标记扩增体系,建立合适的复合扩增体系并以中国东北汉族人群进行验证,对等位基因频率、单倍型数、观察杂合度、有效基因型等相关参数数据进行统计和计算。结果构建了合适的复合扩增体系。经对107个样本验证估计得出等位基因频率,所选择8个DIP-STR遗传标记具有较高的有效基因型概率(I值)。结论8个DIP-STR遗传标记在中国东北汉族人群中具有应用价值。Objective Establishing an appropriate composite amplification system of DIP-STR genetic markers to promote their application into practical cases through verifying them in the Han population of northeast China.Methods Based on the single-genetic marker amplification system designed by Diana Hall et al,a suitable composite amplification system of DIP-STR genetic markers was to build up and verify in the Han population of northeast China.Statistical analysis and calculation were carried out into the relevant data of allelic frequency,haplotype amount,observed heterozygosity and informative genotypes. Results The composite amplification system of DIP-STR genetic markers was successfully established.With the estimation of allelic frequencies through verification from 107 samples into the established composite amplification system,the selected 8 DIPSTR genetic markers had a high probability of informative genotype (I value).Conclusion The eight DIP-STR genetic markers and the involving composite amplification system have forensic applicability in the Han population of northeast China.

关 键 词：DIP-STR 中国东北汉族人群 遗传标记 不平衡DNA 

分 类 号：DF795.2[医药卫生—法医学]