基于锁式探针的荧光定量PCR技术快速检测肉制品中鸭源性成分  

Detection of Duck-derived Ingredients in Meat Products by Realtime Fluorescent PCR Based on Padlock Probe

作　　者：满岳 王溪桥 周小平 Nazariyah Yahaya 丁功涛 李冰 Man Yue;WANG XiQiao;ZHOU XiaoPing;DING GongTao;LI Bing(Gansu Inspection and Quarantine Technology Center,Gansu Entry-Exit Inspection and Quarantine Bureau,Lanzhou 730010,China;FacuLty of Science and Technology,Universiti Sains Islam Malaysia,Nilai,Negeri Sembilan 71800,Malaysia;Northwest Minzu University,Lanzhou 730030,China;Gansu Research Institute of Light Industry,Lanzhou 730000,China)

机构地区：[1]甘肃出入境检验检疫局检验检疫综合技术中心,甘肃兰州730010 [2]马来西亚伊斯兰科技大学科学与技术学院,汝来马来西亚71800 [3]西北民族大学,甘肃兰州730030 [4]甘肃省轻工研究院,甘肃兰州730000

出　　处：《中国食品工业》2019年第4期60-65,共6页China Food Industry

基　　金：甘肃省国际科技合作项目资助,项目号:1604WKCA001.

摘　　要：【目的】肉类掺假是食品行业面临的严峻挑战,其中羊肉和牛肉用鸭肉掺假较为常见。本研究主要目的是为检测掺假肉制品中的鸭源性成分提供一个新的检测方法。【方法】本研究根据鸭线粒体基因组16s rDNA中的特异性序列设计鸭源锁式探针和扩增引物,并结合荧光定量PCR技术,建立肉制品中鸭源性成分检测的新方法。【结果】基于锁式探针的荧光定量PCR技术检测肉制品中鸭源性成分的检测灵敏度达到70fg·μL^-1。【结论】本研究建立的基于锁式探针的荧光定量PCR检测鸭源性成分方法检测特异性强,灵敏度高,为肉类掺假鉴定提供了新的技术手段。【Objective】Meat aduLteration is one of the most common frauduLences in food industry.Counterfeit of the meat product can be sampled as using poor quality products such as duck instead beef or mutton.Identification of animal species will provide a usefuL approach for supervision of aduLteration.【Method】Real-time PCR is the most powerfuL approach used to detect ingredients in meat products based on specific genes.Here,we developed a novel method to visually identify the aduLteration of meat by detecting duck 16s rDNA using real-time PCR based on padlock probe.【ResuLt】ResuLts showed that real-time PCR based on padlock probe specifically identifies duck-derived ingredients,and the limit of detection was 70 fg^-1μL^-1 per reaction.【Conclusion】All these together suggested that real-time PCR based on padlock probe can detected duck-derived ingredients specifically and sensitively,which shouLd be applied in meat aduLteration.

关 键 词：锁式探针 荧光定量PCR 鸭源性成分 肉类掺 

分 类 号：O657.3[理学—分析化学] TS251.7[理学—化学]